FIGURE

Fig. 2

ID

ZDB-FIG-110527-2

Publication

Krueger et al., 2011 - Flt1 acts as a negative regulator of tip cell formation and branching morphogenesis in the zebrafish embryo

Other Figures

All Figure Page

Back to All Figure Page

Fig. 2

Flt1 regulates segmental vessel branching morphogenesis. (A,B) Reduced mFlt1 and sFlt1 protein levels (A) and excessive segmental vessel branching (B) in zebrafish flt1 morphants. The red arrowheads in B indicate the aberrant connection between adjacent segmental vessels. (C,D) Injection of mRNA encoding mflt1 or sflt1 rescued segmental branching defects in flt1 morphants. MO, flt1 ATG morpholino. y-axis shows percentage of examined embryos with aberant segmental vessel branching, as shown in B (lower middle panel; 120 embryos/group from four separate experiments). (E,F) Overexpression of mflt1 (top row) or sflt1 (bottom row) in control embryos results in short segmental artery sprouts (E, left) and reduced filopodia extensions (E, right). The boxed regions are shown at higher magnification in the right-hand panels. y-axis shows percentage of embryos with reduced segmental vessel sprouts, as shown in E. **, P<0.01; ***, P<0.001; Student′s t-test. Error bars indicate s.e.m. (120 embryos/group from three separate experiments).

Expression Data

Gene:	EGFP
Fish:	y1Tg y1Tg + MO3-flt1
Knockdown Reagent:	MO3-flt1
Anatomical Terms:	dorsal aorta dorsal longitudinal anastomotic vessel intersegmental vessel
Stage Range:	Prim-15 to Protruding-mouth

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	y1Tg + MO3-flt1
Knockdown Reagent:	MO3-flt1
Observed In:	blood vessel morphogenesis intersegmental vessel sprouting angiogenesis
Stage Range:	Prim-15 to Protruding-mouth

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Development