ZFIN ID: ZDB-FIG-110527-2
Krueger et al., 2011 - Flt1 acts as a negative regulator of tip cell formation and branching morphogenesis in the zebrafish embryo. Development (Cambridge, England)   138(10):2111-2120 Full text @ Development
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Gene:
Fish:
Knockdown Reagent:
Anatomical Terms:
Stage Range: Prim-15 to Protruding-mouth
PHENOTYPE:
Fish:
Knockdown Reagent:
Observed In:
Stage Range: Prim-15 to Protruding-mouth

Fig. 2 Flt1 regulates segmental vessel branching morphogenesis. (A,B) Reduced mFlt1 and sFlt1 protein levels (A) and excessive segmental vessel branching (B) in zebrafish flt1 morphants. The red arrowheads in B indicate the aberrant connection between adjacent segmental vessels. (C,D) Injection of mRNA encoding mflt1 or sflt1 rescued segmental branching defects in flt1 morphants. MO, flt1 ATG morpholino. y-axis shows percentage of examined embryos with aberant segmental vessel branching, as shown in B (lower middle panel; 120 embryos/group from four separate experiments). (E,F) Overexpression of mflt1 (top row) or sflt1 (bottom row) in control embryos results in short segmental artery sprouts (E, left) and reduced filopodia extensions (E, right). The boxed regions are shown at higher magnification in the right-hand panels. y-axis shows percentage of embryos with reduced segmental vessel sprouts, as shown in E. **, P<0.01; ***, P<0.001; Student′s t-test. Error bars indicate s.e.m. (120 embryos/group from three separate experiments).

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
EGFP y1Tg standard conditions Prim-15 dorsal aorta IFL
Prim-15 intersegmental vessel IFL
Long-pec dorsal aorta IFL
Long-pec dorsal longitudinal anastomotic vessel IFL
Long-pec intersegmental vessel IFL
Protruding-mouth dorsal aorta IFL
Protruding-mouth dorsal longitudinal anastomotic vessel IFL
Protruding-mouth intersegmental vessel IFL
y1Tg + MO3-flt1 standard conditions Prim-15 dorsal aorta IFL
Prim-15 intersegmental vessel IFL
Long-pec dorsal aorta IFL
Long-pec dorsal longitudinal anastomotic vessel IFL
Long-pec intersegmental vessel IFL
Protruding-mouth dorsal aorta IFL
Protruding-mouth dorsal longitudinal anastomotic vessel IFL
Protruding-mouth intersegmental vessel IFL
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
y1Tg + MO3-flt1 standard conditions Prim-15 blood vessel morphogenesis disrupted, abnormal
Prim-15 intersegmental vessel disorganized, abnormal
Prim-15 sprouting angiogenesis disrupted, abnormal
Long-pec blood vessel morphogenesis disrupted, abnormal
Long-pec intersegmental vessel disorganized, abnormal
Long-pec sprouting angiogenesis disrupted, abnormal
Protruding-mouth blood vessel morphogenesis disrupted, abnormal
Protruding-mouth intersegmental vessel disorganized, abnormal
Protruding-mouth sprouting angiogenesis disrupted, abnormal
Acknowledgments:
This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Development