ZFIN ID: ZDB-FIG-110527-3
Krueger et al., 2011 - Flt1 acts as a negative regulator of tip cell formation and branching morphogenesis in the zebrafish embryo. Development (Cambridge, England)   138(10):2111-2120 Full text @ Development
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Gene:
Fish:
Knockdown Reagent:
Anatomical Terms:
Stage Range: Prim-15 to High-pec
PHENOTYPE:
Fish:
Knockdown Reagent:
Observed In:
Stage Range: Prim-15 to High-pec

Fig. 3 Flt1 regulates endothelial tip cell formation. (A,B) Time-lapse in vivo imaging shows increased tip cell numbers and proliferating endothelial tip cells in segmental vessels of zebrafish flt1 morphants. Note that in flt1 morphants the leading cell number 1 gives rise to two progeny cells termed 1.1 and 1.2. y-axis shows the percentage of examined flt1 morphants with proliferation in tip or stalk cells. (C) Quantification of endothelial cell numbers in four consecutive segmental vessel (ISV) – dorsal longitudinal anastomotic vessel (DLAV) loops reveals an increase in flt1 morphants. (D) Anti-phospho-histone H3 staining confirms endothelial proliferation in segmental vessels of flt1 morphants. **, P<0.01; Student′s t-test. Error bars indicate s.e.m.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
EGFP y1Tg standard conditions Prim-15 dorsal aorta IFL
Prim-15 intersegmental vessel IFL
Prim-25 dorsal aorta IFL
Prim-25 dorsal longitudinal anastomotic vessel IFL
Prim-25 intersegmental vessel IFL
High-pec dorsal aorta IFL
High-pec dorsal longitudinal anastomotic vessel IFL
High-pec intersegmental vessel IFL
y1Tg + MO3-flt1 standard conditions Prim-15 dorsal aorta IFL
Prim-15 intersegmental vessel IFL
Prim-25 dorsal aorta IFL
Prim-25 dorsal longitudinal anastomotic vessel IFL
Prim-25 intersegmental vessel IFL
High-pec dorsal aorta IFL
High-pec dorsal longitudinal anastomotic vessel IFL
High-pec intersegmental vessel IFL
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
y1Tg + MO3-flt1 standard conditions Prim-15 blood vessel endothelial cell increased amount, abnormal
Prim-15 blood vessel morphogenesis disrupted, abnormal
Prim-15 intersegmental vessel disorganized, abnormal
Prim-15 sprouting angiogenesis disrupted, abnormal
Prim-15 vascular sprouts has extra parts of type endothelial tip cell, abnormal
Prim-25 blood vessel endothelial cell increased amount, abnormal
Prim-25 blood vessel morphogenesis disrupted, abnormal
Prim-25 intersegmental vessel disorganized, abnormal
Prim-25 sprouting angiogenesis disrupted, abnormal
Prim-25 vascular sprouts has extra parts of type endothelial tip cell, abnormal
High-pec blood vessel endothelial cell increased amount, abnormal
High-pec blood vessel morphogenesis disrupted, abnormal
High-pec intersegmental vessel disorganized, abnormal
High-pec sprouting angiogenesis disrupted, abnormal
High-pec vascular sprouts has extra parts of type endothelial tip cell, abnormal
Acknowledgments:
This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Development