FIGURE

Fig. S6

ID

ZDB-FIG-110516-49

Publication

Lazic et al., 2011 - Mef2cb regulates late myocardial cell addition from a second heart field-like population of progenitors in zebrafish

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Fig. S6

mef2cb morphants showed heart defects that are partially rescued by injection of morpholino-resistant mRNA. (A) 48 hpf heart phenotypes of WT control, mef2cb MO, and MO + mef2cb RNA rescue embryos. WT control embryos showed normal chamber inflation and alignment in 100% of cases (n = 16). Injection of 0.5 ng of mef2cb morpholino resulted in equal proportion of embryos showing strung out hearts (n = 14) and dysmorphic/unlooped hearts (n = 15). Co-injection of morphant embryos with 20 pg of mef2cb morpholino-resistant mRNA resulted in an increase in the milder dysmorphic/unlooped heart phenotype (n = 34) compared to strung out hearts (n = 11). (B–D) Representative images of 48 hpf hearts: (B) WT heart with inflated and aligned atrium and ventricle, (C) strung out heart with no chamber inflation, (D) dysmorphic/unlooped heart with inflated atrium and collapsed ventricle. (E and F) amhc staining in 26 hpf WT control (E) and mef2cb morphant (F).

Expression Data

Gene:	myh6
Fish:	WT WT + MO1-mef2cb
Knockdown Reagent:	MO1-mef2cb
Anatomical Term:	presumptive atrium heart tube
Stage:	Prim-5

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	WT + MO1-mef2cb
Knockdown Reagent:	MO1-mef2cb
Observed In:	atrium cardiac muscle cell differentiation embryonic heart tube morphogenesis heart heart contraction
Stage Range:	Prim-5 to Long-pec

Phenotype Detail

Acknowledgments

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Reprinted from Developmental Biology, 354(1), Lazic, S., and Scott, I.C., Mef2cb regulates late myocardial cell addition from a second heart field-like population of progenitors in zebrafish, 123-133, Copyright (2011) with permission from Elsevier. Full text @ Dev. Biol.