PUBLICATION

Mef2cb regulates late myocardial cell addition from a second heart field-like population of progenitors in zebrafish

Authors

Lazic, S., and Scott, I.C.

ID

ZDB-PUB-110511-7

Date

2011

Source

Developmental Biology 354(1): 123-133 (Journal)

Registered Authors

Lazic, Savo, Scott, Ian

Keywords

mef2c, heart development, second heart field, zebrafish

MeSH Terms

Pyrroles/pharmacology
Animals
Muscle Proteins/genetics*
Muscle Proteins/metabolism
Mice
Stem Cells/metabolism*
Transcription Factors/genetics
Transcription Factors/metabolism
Zebrafish/embryology
Zebrafish/genetics*
Zebrafish/metabolism
Heart/embryology
In Situ Hybridization
Zebrafish Proteins/genetics*
Zebrafish Proteins/metabolism
Time Factors
Signal Transduction/drug effects
Gene Expression Regulation, Developmental/drug effects
Myocardium/cytology
Myocardium/metabolism*
Embryo, Nonmammalian/embryology
Embryo, Nonmammalian/metabolism
Embryo, Nonmammalian/transplantation
Animals, Genetically Modified

(all 24)

PubMed

21466801 Full text @ Dev. Biol.

Abstract

Two populations of cells, termed the first and second heart field, drive heart growth during chick and mouse development. The zebrafish has become a powerful model for vertebrate heart development, partly due to the evolutionary conservation of developmental pathways in this process. Here we provide evidence that the zebrafish possesses a conserved homolog to the murine second heart field. We developed a photoconversion assay to observe and quantify the dynamic late addition of myocardial cells to the zebrafish arterial pole. We define an extra-cardiac region immediately posterior to the arterial pole, which we term the late ventricular region. The late ventricular region has cardiogenic properties, expressing myocardial markers such as vmhc and nkx2.5, but does not express a full complement of differentiated cardiomyocyte markers, lacking myl7 expression. We show that mef2cb, a zebrafish homolog of the mouse second heart field marker Mef2c, is expressed in the late ventricular region, and is necessary for late myocardial addition to the arterial pole. FGF signaling after heart cone formation is necessary for mef2cb expression, the establishment of the late ventricular region, and late myocardial addition to the arterial pole. Our study demonstrates that zebrafish heart growth shows more similarities to murine heart growth than previously thought. Further, as congenital heart disease is often associated with defects in second heart field development, the embryological and genetic advantages of the zebrafish model can be applied to study the vertebrate second heart field.

Genes / Markers

Figures

Show all Figures

Expression

Gene	Fish	Conditions	Stage	Anatomy	Assay	Figure
KikGR	hsc6Tg	standard conditions	Long-pec	cardiac muscle cell nucleus	IFL	Fig. 1
KikGR	hsc7Tg	standard conditions	Long-pec	heart vascular endothelium	IFL	Fig. 1
KikGR	hsc7Tg	standard conditions	Long-pec	vascular endothelium nucleus	IFL	Fig. 1
mef2ca	WT	standard conditions	20-25 somites	heart rudiment	ISH	Fig. S5
mef2ca	WT	standard conditions	Prim-15	presumptive atrium heart tube	ISH	Fig. S5
mef2ca	WT	standard conditions	Prim-15	presumptive cardiac ventricle heart tube	ISH	Fig. S5
mef2ca	WT	standard conditions	20-25 somites	somite	ISH	Fig. S5
mef2cb	WT	standard conditions	14-19 somites	heart primordium	ISH	Fig. 3
mef2cb	WT	standard conditions	14-19 somites	heart primordium	ISH	Fig. 4
mef2cb	WT	standard conditions	26+ somites	heart rudiment	ISH	Fig. 3

1 - 10 of 41

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Phenotype

Fish	Conditions	Stage	Phenotype	Figure
WT	chemical treatment: SU5402	Prim-15	cardiac ventricle decreased size, abnormal	Fig. 6
WT	chemical treatment: SU5402	Prim-15	embryonic heart tube morphogenesis process quality, abnormal	Fig. 6
WT + MO1-mef2cb	standard conditions	14-19 somites	cardiac muscle cell differentiation process quality, abnormal	Fig. 4
WT + MO1-mef2cb	standard conditions	20-25 somites	cardiac muscle cell differentiation process quality, abnormal	Fig. 4
WT + MO1-mef2cb	standard conditions	Prim-5	atrium physical object quality, abnormal	Fig. S6
WT + MO1-mef2cb	standard conditions	Prim-5	cardiac muscle cell differentiation process quality, abnormal	Fig. 4
WT + MO1-mef2cb	standard conditions	Prim-5	cardiac muscle cell differentiation process quality, abnormal	Fig. S6
WT + MO1-mef2cb	standard conditions	Prim-15	cardiac ventricle decreased size, abnormal	Fig. 4
WT + MO1-mef2cb	standard conditions	Long-pec	embryonic heart tube morphogenesis process quality, abnormal	Fig. S6
WT + MO1-mef2cb	standard conditions	Long-pec	heart contraction decreased rate, abnormal	Fig. S6

1 - 10 of 14

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Mutations / Transgenics

Allele	Construct	Type
hsc6Tg	Tg(myl7:NLS-KikGR)	Transgenic Insertion
hsc7Tg	Tg(kdrl:NLS-KikGR)	Transgenic Insertion
twu34Tg	Tg(myl7:EGFP)	Transgenic Insertion

Human Disease / Model

Sequence Targeting Reagents

Fish

Antibodies

Orthology

Engineered Foreign Genes

Marker	Marker Type	Name
EGFP	EFG	EGFP
KikGR	EFG	KikGR

Mapping

Lazic et al., 2011 ZDB-PUB-110511-7 PMID:21466801

Mef2cb regulates late myocardial cell addition from a second heart field-like population of progenitors in zebrafish

Lazic et al., 2011

ZDB-PUB-110511-7
PMID:21466801