FIGURE

Fig. 10

ID

ZDB-FIG-110324-34

Publication

Sheets et al., 2011 - Ribeye is required for presynaptic CaV1.3a channel localization and afferent innervation of sensory hair cells

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Fig. 10

Ectopic aggregates of overexpressed Ribeye colocalize with ectopic clusters of Ca_V1.3a channel in hair cells. Merged images include DAPI (blue). Scale bars: 3 μm. (A,A2) Ribeye b (green) and Ca_V1.3a (red) immunolabeling in cross-sections of anterior lateral line neuromasts in 6 dpf larvae. (B) RT-PCR analysis of cav1.3a transcripts in wild-type and transgenic larvae. gapdh was used as a loading control. (C,C2) Ribeye b (green) and MAGUK (red) immunofluorescent labeling in cross-sections of neuromasts. (D) The average number of MAGUK-positive punctae in wild-type and larvae stably expressing high levels of GFP-tagged Ribeye b. Error bars indicate s.e.m.

Expression Data

Genes:	cacna1da ctbp2l
Antibodies:	Ab1-cacna1da Ab1-pan-MAGUK Ab3-ctbp2l
Fish:	vo67Tg
Anatomical Terms:	anterior lateral line nerve postsynaptic density anterior lateral line neuromast neuromast hair cell neuromast hair cell presynaptic active zone
Stage:	Day 6

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	vo67Tg
Observed In:	neuromast hair cell neuromast hair cell cytoskeleton of presynaptic active zone neuromast hair cell voltage-gated calcium channel complex presynaptic membrane assembly
Stage:	Day 6

Phenotype Detail

Acknowledgments

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