ZFIN ID: ZDB-FIG-110324-30
Sheets et al., 2011 - Ribeye is required for presynaptic CaV1.3a channel localization and afferent innervation of sensory hair cells. Development (Cambridge, England)   138(7):1309-1319 Full text @ Development
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Antibody:
Fish:
Knockdown Reagents:
Anatomical Terms:
Stage: Day 4
PHENOTYPE:
Fish:
Knockdown Reagents:
Observed In:
Stage: Day 4

Fig. 6

Depletion of Ribeye results in reduced afferent innervation. (A) Representative confocal z-projections showing GFP-labeled innervating afferent neurons (green), Ribeye a immunolabel (red) and DAPI label (blue) in lateral line neuromast 2 from three individual 4 dpf larva. Scale bars: 3 ┬Ám. (B,C) Plots of the relationship between either the total length of innervating afferent processes (B) or the number of afferent branch junctions (C) and the number of Ribeye a immunolabeled punctae in ribeye a and ribeye b morphants. Each point represents neuromast 2 in an individual larva. (D,E) Plot of the relationships between the total length of innervating afferent processes (D) or the number of afferent branch junctions (E) and the number of Ribeye a immunolabeled punctae in wild-type and control MO-injected larvae. There was no significant correlation between dendritic length (Pearson′s r=0.4525; P=0.08) or the number of afferent branches (Pearson′s r=0.0129; P=0.97) and the number of Ribeye a punctae. The linear regressions of the plots in either B or C are included for comparison (broken red line).

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
ctbp2a Ab1-ctbp2a nl1Tg control Day 4 neuromast hair cell cytoskeleton of presynaptic active zone IHC
Ab1-ctbp2a nl1Tg + MO1-ctbp2a + MO2-ctbp2l standard conditions Day 4 neuromast hair cell cytoskeleton of presynaptic active zone IHC
EGFP nl1Tg control Day 4 lateral line nerve dendrite IFL
Day 4 posterior lateral line nerve dendrite IFL
nl1Tg + MO1-ctbp2a + MO2-ctbp2l standard conditions Day 4 lateral line nerve dendrite IFL
Day 4 posterior lateral line nerve dendrite IFL
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
nl1Tg + MO1-ctbp2a + MO2-ctbp2l standard conditions Day 4 axonogenesis involved in innervation decreased process quality, abnormal
Day 4 neuromast hair cell cytoskeleton of presynaptic active zone decreased amount, abnormal
Day 4 posterior lateral line nerve dendrite decreased length, abnormal
Day 4 posterior lateral line nerve dendrite unbranched, abnormal
Acknowledgments:
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