Fig. S2

Spatial expression of zebrafish hmgb1a. (A–C) At early developmental stages zebrafish hmgb1a is ubiquitously expressed. (D and E) At 6–8 somites it is preferentially expressed in somites subdomains (D, white arrow) and at 20–22 somites it is still broadly expressed, with the exception of the notochord and somites (E, grey arrow). (F and G) At 24 hpf, hmgb1a expression is restricted to specific regions of the embryos, mainly in the CNS, but is also visible in the spinal cord (SC), in the lateral line primordium (F, blue arrow), in the intermediate cell mass (F, blue arrowhead) and in pectoral fin buds (G, asterisk). (H and I) At 2 dpf the detectable signal is in the rostral CNS, in lateral line neuromasts (H, arrowheads), in the gut (H, arrow) and in pectoral fin buds (I, asterisk). Panel D shows a flat-mount embryo; all other panels show whole-mount images. (A, C, D, G and I) Dorsal views; (B, E, F and H) lateral views, anterior to the left. All images are taken at 40× magnification. Pictures showing shortly stained embryos were chosen when expression details could not be clearly visualized upon prolonged staining (panels F and G). No significant signal was obtained in ISHs performed with a sense probe (data not shown).