Fig. S6

Spatial expression of zebrafish hmgb3a. (A–C) At early developmental stages hmgb3a is ubiquitously expressed, and at tailbud stage it is particularly expressed at the midline (C, white arrowhead). (D–G) Even during somitogenesis it is broadly expressed, with the exception of notochord, somites (F, grey arrow) and eyes (G, white asterisk). (H and I) At 24 hpf, hmgb3a expression is restricted to specific regions of the embryos, mainly in the CNS, but is also visible in the spinal cord (SC), in the lateral line primordium (H, blue arrow), in the intermediate cell mass (H, blue arrowhead) and weakly in pectoral fin buds (I, asterisk). (L–M) At 2 dpf the signal is detectable in the rostral CNS and weakly in pectoral fin buds (M, asterisk). All panels show whole-mount images. (A, C, E, G, I and M) Dorsal views; (B, D, F, H and L) lateral views, anterior to the left. All images are taken at 40× magnification. Prolonged staining was performed at all stages to better visualize the expression pattern. No significant signal was obtained in ISHs performed with a sense probe (data not shown).