Fig. S5

Spatial expression of zebrafish hmgb2b. (A–C) At early developmental stages hmgb2b is highly and ubiquitously expressed, and at tailbud stage it is particularly expressed at the midline (C, white arrowhead). (D–E) Even during somitogenesis it is broadly expressed, with the exception of notochord and somites (E, grey arrow). (F and G) At 24 hpf, hmgb2b is highly and broadly expressed, with the notable exception of the somitic mesoderm and notochord. (H and I) At 2 dpf the detectable signal is in the rostral CNS, in pectoral fin buds, in lateral line neuromasts (H, arrowheads) and in the gut (H, arrow). All panels show whole-mount images. (A, C, G and I) Dorsal views; (B, D, E, F and H) lateral views, anterior to the left. All images are taken at 40× magnification. Pictures showing shortly stained embryos were chosen when expression details could not be clearly visualized upon prolonged staining (panels E–G). No significant signal was obtained in ISHs performed with a sense probe (data not shown).