FIGURE

Fig. 3

ID

ZDB-FIG-110317-13

Publication

Osborn et al., 2011 - Cdkn1c drives muscle differentiation through a positive feedback loop with Myod

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Fig. 3

Hh causes Cdkn1c to stabilize Myod. (A). Cdkn1c mRNA is Hh-dependent in adaxial cells. Shha^tbx392 or smo^b641 mutation or cyA treatment reduces cdkn1c mRNA. Note that loss was proportional to reduction in Hh signal (arrowheads and black bracket). Shha mRNA injection caused unilateral somitic cdkn1c up-regulation (red bracket). (B,C). Cdkn1c 5′ MO injection into embryos from a shha^tbx392/+ incross reduced adaxial myog mRNA (B), Myod or MyHC proteins (C). Note the delay in myog mRNA and reduction in Myod protein (brackets). (D). Cdkn1c ATG MO injection reduces adaxial Myod at tailbud stage. Bar: 100 μm.

Expression Data

Genes:	cdkn1ca myf5 myod1 myog
Antibodies:	Ab-A4.1025 Ab1-myod1
Fish:	WT shha^{tbx392/tbx392} smo^b641/b641 WT + MO2-cdkn1ca WT + MO3-cdkn1ca shha^{tbx392/tbx392} + MO3-cdkn1ca
Condition:	chemical treatment: Cyclopamine
Knockdown Reagents:	MO2-cdkn1ca MO3-cdkn1ca
Anatomical Terms:	adaxial cell segmental plate somite
Stage Range:	Bud to Prim-5

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	shha^{tbx392/tbx392} WT + MO2-cdkn1ca WT + MO3-cdkn1ca shha^{tbx392/tbx392} + MO3-cdkn1ca
Knockdown Reagents:	MO2-cdkn1ca MO3-cdkn1ca
Observed In:	adaxial cell
Stage Range:	Bud to 5-9 somites

Phenotype Detail

Acknowledgments

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Reprinted from Developmental Biology, 350(2), Osborn, D.P., Li, K., Hinits, Y., and Hughes, S.M., Cdkn1c drives muscle differentiation through a positive feedback loop with Myod, 464-475, Copyright (2011) with permission from Elsevier. Full text @ Dev. Biol.