ZFIN Image: Osborn et al., 2011, Fig. 3

Image

Figure Caption

Fig. 3 Hh causes Cdkn1c to stabilize Myod. (A). Cdkn1c mRNA is Hh-dependent in adaxial cells. Shha^tbx392 or smo^b641 mutation or cyA treatment reduces cdkn1c mRNA. Note that loss was proportional to reduction in Hh signal (arrowheads and black bracket). Shha mRNA injection caused unilateral somitic cdkn1c up-regulation (red bracket). (B,C). Cdkn1c 5′ MO injection into embryos from a shha^tbx392/+ incross reduced adaxial myog mRNA (B), Myod or MyHC proteins (C). Note the delay in myog mRNA and reduction in Myod protein (brackets). (D). Cdkn1c ATG MO injection reduces adaxial Myod at tailbud stage. Bar: 100 μm.

Figure Data

Expression / Labeling details

Phenotype details

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image.

Reprinted from Developmental Biology, 350(2), Osborn, D.P., Li, K., Hinits, Y., and Hughes, S.M., Cdkn1c drives muscle differentiation through a positive feedback loop with Myod, 464-475, Copyright (2011) with permission from Elsevier. Full text @ Dev. Biol.