ZFIN ID: ZDB-FIG-110207-61
Yu et al., 2011 - Cilia-driven fluid flow as an epigenetic cue for otolith biomineralization on sensory hair cells of the inner ear. Development (Cambridge, England)   138(3):487-494 Full text @ Development
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Antibody:
Fish:
Condition:
Knockdown Reagents:
Anatomical Terms:
Stage Range: Bud to 20-25 somites

Fig. 6 Atoh1b-dependent regulation of foxj1b in kinocilia differentiation. (A) Electron micrograph (transverse section) of kinocilia (arrows) from the ear of a mib mutant zebrafish embryo. The microvilli (stereocilia) of the hair cells are also visible (small arrows). (B) High-resolution image showing the 9+2 arrangement of microtubules. The central pair of microtubules is indicated (arrowheads). (C) Wild-type pattern of atoh1b expression in the otic vesicle of a foxj1b morphant (compare with Fig. 3E). (D) Normal pattern of foxj1b expression in the otic placode of an atoh1b morphant at 10 hpf (compare with Fig. 1A). (E) Loss of foxj1b expression (arrows) from the ear of an atoh1b morphant at 16 hpf (compare with Fig. 1B). (F) Expression of pou4f3 in the differentiating hair cells (arrows) of a wild-type ear. (G) Ectopic pou4f3 expression (arrows) in supernumerary hair cells in the ear of a Tg(hs::atoh1b) transgenic embryo. (H) Ectopic foxj1b expression (arrows) in supernumerary hair cells in the ear of a Tg(hs::atoh1b) transgenic embryo (compare with Fig. 1C). (I) Kinocilia (small arrows) on ectopic hair cells (long arrows) in the ear of a Tg(hs::atoh1b) transgenic embryo. The embryo was stained with antibodies to acetylated tubulin (green) and with DAPI (blue). Scale bar: 10 μm in I.

Gene Expression Details
Gene Antibody Fish Conditions Stage Qualifier Anatomy Assay
atoh1b WT + MO3-foxj1b standard conditions 14-19 somites otic vesicle ISH
foxj1b WT + MO1-atoh1b standard conditions Bud otic placode ISH
14-19 somites Not Detected otic vesicle ISH
sq5711Tg heat shock 20-25 somites otic vesicle ISH
pou4f3 WT standard conditions 14-19 somites auditory receptor cell ISH
sq5711Tg heat shock 20-25 somites otic vesicle ISH
Antibody Labeling Details
Antibody Assay Fish Conditions Stage Anatomy
Ab1-tuba IHC sq5711Tg heat shock 20-25 somites auditory receptor cell kinocilium
Phenotype Details No data available
Acknowledgments:
ZFIN wishes to thank the journal Development (Cambridge, England) for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Development