FIGURE

Fig. 4

ID
ZDB-FIG-110207-59
Publication
Yu et al., 2011 - Cilia-driven fluid flow as an epigenetic cue for otolith biomineralization on sensory hair cells of the inner ear
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Fig. 4

Foxj1b is required for the differentiation of motile and kinocilia. (A) Expression of GFP in the hair cells of a heterozygous T2BGSZ10 transgenic zebrafish embryo. Arrows indicate hair cells. (B) Defective motile cilia differentiation in the ear of a homozygous T2BGSZ10 transgenic embryo (compare with Fig. 1E). (C) Defective kinocilia differentiation in the ear of a homozygous T2BGSZ10 transgenic embryo. Note the variable lengths of the kinocilia (arrows) (compare with Fig. 1F). (D) Malformed otoliths (green, arrows) in the ear of a homozygous T2BGSZ10 transgenic embryo. (E) A foxj1b morpholino-injected homozygous T2BGSZ10 transgenic embryo showing a more pronounced effect on ciliary differentiation with severe reduction in the motile and kinocilia. (F) A more prominent effect on otolith (green, arrows) formation in the ear of a foxj1b morpholino-injected homozygous T2BGSZ10 transgenic embryo. Embryos in A-C,E were stained with anti-acetylated tubulin antibodies (red), and those in D and F with anti-Stm antibodies (green). The embryo in A was co-stained with anti-GFP antibodies (green), whereas those in D and F were co-stained with anti-β-catenin antibodies (red). Nuclei were visualized with DAPI (blue). All panels show lateral views of otic vesicles with anterior to the left. Scale bars: 10 μm.

Expression Data
Gene:
Antibodies:
Fish:
Knockdown Reagent:
Anatomical Terms:
Stage Range: 14-19 somites to Prim-5

Expression Detail
Antibody Labeling
Phenotype Data
Fish:
Knockdown Reagent:
Observed In:
Stage: Prim-5

Phenotype Detail
Acknowledgments
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