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Fig. 2 Spry4 loss-of-function results in hindbrain patterning defects. (A-G) Zebrafish embryos injected with either control morpholino (Ctrlmo) (A,F), Spry1mo (B), Spry2mo (C), Spry4mo (D,G) or both Spry1mo and Spry2mo (E) were collected at the 10-somite (A-E) or 12-somite (F,G) stage and subjected to in situ hybridisation for krox20 and her5, a marker of the MHB (A-E, both purple), or for krox20 (red), her5 and hoxb1a (purple) (F,G). Embryos are flat-mounted with anterior to the left. (H,I) Quantitative evaluation of rhombomere areas. Normalised areas were obtained by dividing each rhombomere area by one fifth of the area of the neural plate from r1 to r5. ns, not significant (P>0.05); *, P<0.004; **, P<0.0001; Student′s t-test. Errors bars indicate s.e.m.