ZFIN ID: ZDB-FIG-101118-32
Song et al., 2010 - Neural and Synaptic Defects in slytherin, a Zebrafish Model for Human Congenital Disorders of Glycosylation. PLoS One   5(10):e13743 Full text @ PLoS One
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Antibody:
Fish:
Knockdown Reagent:
Anatomical Term:
Stage: Protruding-mouth
PHENOTYPE:
Fish:
Knockdown Reagent:
Observed In:
Stage: Protruding-mouth

Fig. 6 A. AAL staining is reduced in srn, gmds morphants and gmds morphants overexpressing NICD, but not in WT embryos or WT embryos overexpressing NICD. B. WT and WT overexpressing NICD had normal Zn5+ cell patterning. srn and gmds MO showed Zn5+ cell patterning defects which was rescued by NICD overexpression in gmds morphants. C. WT and WT overexpressing NICD had normal GFAP+ glia cells in spinal cord. srn and gmds morphants had reduced GFAP+ glia cells, rescued by NICD overexpression in gmds morphants (>10 embryos in each experiment). Scale bar = 40 μm. Dā€“E. Quantification of Zn5+ cell patterning defects. There are 3ā€“5 Zn5+ cells every 20 μm in WT, WT overexpressing NICD and gmds MO overexpressing NICD; compared to 1ā€“8 in srn and gmds MO embryos. D, data from a representative embryo; E, distribution of all embryos (4ā€“6 embryos; Kolmogorov-Smirnov test, * p<0.05).

Gene Expression Details No data available
Antibody Labeling Details
Antibody Assay Fish Conditions Stage Anatomy
zn-5 IHC WT + MO2-gmds standard conditions Protruding-mouth secondary motor neuron
IHC gmdsp31erb/p31erb standard conditions Protruding-mouth secondary motor neuron
IHC kca3Tg; kca4Tg standard conditions Protruding-mouth secondary motor neuron
IHC kca3Tg; kca4Tg + MO2-gmds standard conditions Protruding-mouth secondary motor neuron
Phenotype Details
Fish Conditions Stage Phenotype
WT + MO2-gmds standard conditions Protruding-mouth axon guidance disrupted, abnormal
Protruding-mouth secondary motor neuron axon morphology, abnormal
Protruding-mouth secondary motor neuron decreased amount, abnormal
Protruding-mouth spinal cord glial cell decreased amount, abnormal
gmdsp31erb/p31erb standard conditions Protruding-mouth axon guidance disrupted, abnormal
Protruding-mouth secondary motor neuron axon morphology, abnormal
Protruding-mouth secondary motor neuron decreased amount, abnormal
Protruding-mouth spinal cord glial cell decreased amount, abnormal
kca3Tg; kca4Tg standard conditions Protruding-mouth secondary motor neuron amount, normal
Protruding-mouth secondary motor neuron axon morphology, normal
Protruding-mouth secondary motor neuron spatial pattern, normal
kca3Tg; kca4Tg + MO2-gmds standard conditions Protruding-mouth secondary motor neuron amount, normal
Protruding-mouth secondary motor neuron axon morphology, normal
Protruding-mouth secondary motor neuron spatial pattern, normal
Acknowledgments:
ZFIN wishes to thank the journal PLoS One for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ PLoS One