ZFIN ID: ZDB-FIG-101108-47
Poulton et al., 2010 - A novel role for Glucocorticoid-Induced TNF Receptor Ligand (Gitrl) in early embryonic zebrafish development. The International journal of developmental biology   54(5):815-825 Full text @ Int. J. Dev. Biol.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Knockdown Reagent:
Anatomical Term:
Stage Range: 30%-epiboly to Prim-5
PHENOTYPE:
Fish:
Knockdown Reagents:
Observed In:
Stage Range: Shield to 14-19 somites

Fig. 7 Gitrl and the Stat3-Liv1-Cdh1 axis. (A) Transcription of liv1 and cdh1 were assessed by real-time PCR at various time points during gastrulation and segmentation in embryos injected with 10 ng of either control1 or zGL1. Values were normalised to ef1α and levels in zGL1 injected embryos expressed relative to levels detected in control-injected embryos using the ΔΔCt method (ABI Prism 7700 Sequence Detection System, user bulletin no. 2). Mean values ± SD of three independent experiments are shown. Embryos injected with either (B,F) 10 ng of control 1 + 2.5 ng of cdh1 MO control (revcdh1), (C,G) 10 ng of zGL1 + 2.5 ng revcdh1, (D,H) 10 ng of zGL1 + 2.5 ng of cdh1 MO (cdh1) or (E,I) 10 ng of control 1 + 2.5 ng of cdh1 were observed at (B-E) 6 and (F-I) 8 hpf. Embryos treated with control MOs only were indistinguishable from wild-type embryos. Those treated with the zGL1 plus revcdh1 showed the characteristics of gitrl morphants previously described, and embryos injected with cdh1 MO plus control1 resembled the avalanche mutant (Kane et al., 1996). Surprisingly, embryos co-injected with both gitrl and cdh1 MOs had a novel phenotype characterized by arrest during the blastula period and a complete failure to enter epiboly. The phenotype shown in (D) and (H) persisted until the embryos died at approximately 15 hpf.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
cdh1 WT control 30%-epiboly to Prim-5 whole organism RTPCR
WT + MO1-tnfsf18 standard conditions 30%-epiboly to Prim-5 whole organism RTPCR
slc39a6 WT control 30%-epiboly to Prim-5 whole organism RTPCR
WT + MO1-tnfsf18 standard conditions 30%-epiboly to Prim-5 whole organism RTPCR
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
WT + MO1-tnfsf18 standard conditions Shield - 75%-epiboly epiboly involved in gastrulation with mouth forming second delayed, abnormal
Shield - 14-19 somites regulation of transcription, DNA-templated disrupted, abnormal
WT + MO1-tnfsf18 + MO6-cdh1 standard conditions Shield - 75%-epiboly embryonic cleavage arrested, abnormal
Shield - 75%-epiboly epiboly involved in gastrulation with mouth forming second arrested, abnormal
WT + MO6-cdh1 standard conditions Shield - 75%-epiboly epiboly involved in gastrulation with mouth forming second arrested, abnormal
Acknowledgments:
ZFIN wishes to thank the journal The International journal of developmental biology for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Int. J. Dev. Biol.