ZFIN ID: ZDB-FIG-101108-46
Poulton et al., 2010 - A novel role for Glucocorticoid-Induced TNF Receptor Ligand (Gitrl) in early embryonic zebrafish development. The International journal of developmental biology   54(5):815-825 Full text @ Int. J. Dev. Biol.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Gene:
Antibody:
Fish:
Knockdown Reagent:
Anatomical Term:
Stage Range: Shield to 20-25 somites
PHENOTYPE:
Fish:
Knockdown Reagent:
Observed In:
Stage Range: Shield to 20-25 somites

Fig. 6 Identification of Stat3 phosphorylation as a possible mediator of Gitrl effects during embryogenesis. (A) Total protein lysates were generated from embryos injected with 10 ng of control1 (C) or zGL1 (MO) and harvested at 6, 8, 11 and 19 hpf. The lysates were analysed by western blotting using antibodies specific for phosphorylated (P-Stat3) and total (Stat3) Stat3 content. Embryos (AB*) treated with (B-F) DMSO or (G-K) 5 μM of stattic at 2-4 hpf were compared for phenotypic overlap with the gitrl morphant phenotype shown in figure 3. Stattic-treated embryos displayed incomplete blastoderm margin advancement (arrowhead in (G)) compared to controls (arrowhead in (B)), and indistinct somite boundary formation (H-K) similar to that seen in gitrl morphants. (L) TL embryos were more sensitive than AB* embryos to Stat3 inhibition. (M) TL embryos treated with a low dose of zGL1 (2.5 ng) were additionally treated with either DMSO or 2 mM of stattic at 2 hpf. Embryos that received low-dose MO plus DMSO were viable, but showed incomplete blastoderm margin advancement at 75 % epiboly, while embryos treated with low-dose zGL1 in addition to treatment with stattic showed a lethal phenotype.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
stat3 Ab1-stat3 WT control Shield to 20-25 somites whole organism WB
Ab1-stat3 WT + MO1-tnfsf18 standard conditions Shield to 20-25 somites whole organism WB
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
WT + MO1-tnfsf18 standard conditions Shield - 20-25 somites regulation of tyrosine phosphorylation of STAT protein disrupted, abnormal
Acknowledgments:
ZFIN wishes to thank the journal The International journal of developmental biology for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Int. J. Dev. Biol.