ZFIN ID: ZDB-FIG-101108-42
Poulton et al., 2010 - A novel role for Glucocorticoid-Induced TNF Receptor Ligand (Gitrl) in early embryonic zebrafish development. The International journal of developmental biology   54(5):815-825 Full text @ Int. J. Dev. Biol.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Anatomical Terms:
Stage Range: 1-cell to Adult

Fig. 2 Expression of zebrafish gitrl and girt genes in adult tissues and during development. Real-time PCR was used to assess the expression of gitrl and girt in (A) adult zebrafish tissues and (B) at different time points during embryonic development. Gene expression was quantified using a standard curve method and is shown normalised to ef1α transcript levels and expressed relative to levels in adult kidney. Values are shown as mean ± SD of technical triplicates for an individual experiment representative of three biological replicates. In adult tissues girt mRNA was not detected in brain or ovary. In embryos, only gitrl is shown as no girt mRNA was detected during this period. (C) Localisation of gitrl transcripts at different time-points within developing embryos was investigated by in situ hybridization using a DIG-labelled antisense RNA probe. Distinct bands along the paraxial mesoderm can be seen at 10 hpf, with localization of expression to the head region by 26 hpf. A control embryo processed at 16 hpf in the absence of probe is shown to indicate the level of background staining and any yolk trapping.

Gene Expression Details
Gene Antibody Fish Conditions Stage Qualifier Anatomy Assay
tnfrsf18 WT standard conditions Adult Not Detected brain RTPCR
Adult gut RTPCR
Adult kidney RTPCR
Adult Not Detected ovary RTPCR
Adult spleen RTPCR
Adult thymus RTPCR
tnfsf18 WT standard conditions 1-cell to Prim-25 whole organism RTPCR
Bud paraxial mesoderm ISH
Prim-5 head ISH
Adult brain RTPCR
Adult gut RTPCR
Adult kidney RTPCR
Adult ovary RTPCR
Adult spleen RTPCR
Adult thymus RTPCR
Antibody Labeling Details No data available
Phenotype Details No data available
Acknowledgments:
ZFIN wishes to thank the journal The International journal of developmental biology for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Int. J. Dev. Biol.