FIGURE

Fig. S5

ID

ZDB-FIG-101103-10

Publication

Davidson et al., 2010 - Differential regulation of p53 function by the N-terminal ?Np53 and ?113p53 isoforms in zebrafish embryos

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Fig. S5

Characterization of ?Np53-specific morpholino and effects on target gene expression. (A) Efficacy of ?Np53 knockdown by the morpholino directed against sequences in intron 2 of ?Np53. The effect of ?Np53 MO on a construct containing the 5′ sequences of ?Np53 driving luciferase expression and injected into zebrafish embryos at the 2-4 cell stage was measured and compared to the effects of a mismatch MO control (see Table 1). (B) Effect of ?Np53 MO on p21 and ?113p53 steady-state transcript levels as determined by RT-qPCR in irradiated embryos (IR (20 Gy) at 24 hpf, RNA isolation at 30 hpf). For comparison downregulation of target gene expression by FLp53-directed MO [11] are shown (grey bars). All results are shown were statistically different (p < 0.05; ANOVA-Bonferroni test) relative to mRNA expression levels of unirradiated embryos. Control mock-injected embryos (white bars) received 20 Gy at 24 hpf.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ BMC Dev. Biol.