FIGURE

Fig. 1

ID

ZDB-FIG-101103-1

Publication

Davidson et al., 2010 - Differential regulation of p53 function by the N-terminal ?Np53 and ?113p53 isoforms in zebrafish embryos

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Fig. 1

Molecular attributes of zebrafish ?Np53. (A) RT-PCR analysis of zebrafish ?Np53. The zebrafish ?Np53 transcript was detected in cDNA preparations from irradiated embryos using the intron 2 forward primer (I2) and the exon 5 (E5) reverse primer. (B) Comparative view of human and zebrafish ?Np53 sequences. In zebrafish, the alternatively spliced intron 2 (blue highlight) contains an in frame stop codon (T; red) and an alternate start codon (M2; purple) in frame with the p53 ORF of exon 3. (C) Semi-quantitative RT-PCR analysis of p53 isoform expression 6 h post irradiation of 24 hpf embryos. (D) Microinjection of 5′UTR-?Np53 M2-luciferase mRNA into zebrafish embryos. Strong expression of the luciferase reporter gene was achieved using this expression construct, which was abrogated by changing the putative M2 translation initiation site to glycine.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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