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Fig. S5 Characterization of ?Np53-specific morpholino and effects on target gene expression. (A) Efficacy of ?Np53 knockdown by the morpholino directed against sequences in intron 2 of ?Np53. The effect of ?Np53 MO on a construct containing the 5′ sequences of ?Np53 driving luciferase expression and injected into zebrafish embryos at the 2-4 cell stage was measured and compared to the effects of a mismatch MO control (see Table 1). (B) Effect of ?Np53 MO on p21 and ?113p53 steady-state transcript levels as determined by RT-qPCR in irradiated embryos (IR (20 Gy) at 24 hpf, RNA isolation at 30 hpf). For comparison downregulation of target gene expression by FLp53-directed MO [11] are shown (grey bars). All results are shown were statistically different (p < 0.05; ANOVA-Bonferroni test) relative to mRNA expression levels of unirradiated embryos. Control mock-injected embryos (white bars) received 20 Gy at 24 hpf.