FIGURE

Fig. 3

ID

ZDB-FIG-101103-3

Publication

Davidson et al., 2010 - Differential regulation of p53 function by the N-terminal ?Np53 and ?113p53 isoforms in zebrafish embryos

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Fig. 3

Developmental malformations and hypoplasia caused by ectopic expression of zebrafish ΔNp53 mRNA. (A) Representative images of the head region (upper panel) and quantification (lower panel) of eye size in embryos injected with either zebrafish ?Np53 mRNA or GFP encoding control mRNA (1 ng/embryo). Eye size was determined by outlining the eye using ImageJ software (NIH) and calculating the eye area within the circumference. Results represent the mean � SEM of 5 embryos. The asterisk denotes statistically significant difference (t-test, one tailed; p < 0.01). (B) Representative images of embryos and magnification of somites (upper panel) and quantification (lower panel) of somite size. Somite width was determined using ImageJ software (NIH). Results represent the mean of the width of five somites from three individual fish (mean � SEM; n = 15). Asterices denote statistically significant differences (p < 0.001). The white bar in the GFP mRNA image represents 0.2 mm (10� magnification for all images). In addition to the effects of ?Np53 mRNA the effects of coinjecting a p21-targeted MO are shown.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ BMC Dev. Biol.