FIGURE

Fig. S4

ID

ZDB-FIG-101103-9

Publication

Davidson et al., 2010 - Differential regulation of p53 function by the N-terminal ?Np53 and ?113p53 isoforms in zebrafish embryos

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Fig. S4

Survival and morphological appearance of zebrafish embryo injected with C-terminally truncated ?Np53(?302-374) mRNA. (A) Triplicate dishes of 30 embryos each were injected with mRNA encoding GFP (control), ?Np53 mRNA or ?Np53(?302-374) mRNA lacking the dimerization domain. Survival was determined by the presence of a heartbeat was assessed over 7 days. (B) Upper panel: representative images of zebrafish embryos at 24 hpf. Lower panel: representative images of single embryos at 48 hpf. Bar (lower panel) = 0.5 mm. (C) Injection of ?Np53(?302-374) mRNA did not affect steady-state p21 message levels (grey bar). This is in contrast to dimerization-competent ?Np53 mRNA containing C-terminal sequences corresponding to the p53 α isoform used as a positive control (black bar).

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ BMC Dev. Biol.