FIGURE

Fig. 8

ID

ZDB-FIG-101101-8

Publication

Ablooglu et al., 2010 - Integrin alphaV is necessary for gastrulation movements that regulate vertebrate body asymmetry

Other Figures

All Figure Page

Back to All Figure Page

Fig. 8

KV lumen does not properly form in αV and β1b morphants. Confocal images of Tg(sox17:GFP)-expressing embryos (green) are shown. (A-C,G-I) Single focal planes at the center of the DFC cluster immunolabeled with anti-ZO-1 antibody (red; A-C) or anti-aPKC-ζ antibody (red) and anti-acetylated tubulin antibody (white; G-I). (D-F) 3D renderings of anti-ZO-1 labeled embryos of A-C. Dorsal views of 6-8 SS embryos are shown in all panels, anterior to the top. Embryos injected with αV1miss control MO developed a large fluid-filled lumen (A,G) that had a uniform ZO-1-labeled tight junction lattice within the DFC-derived lining of the KV (D). However, in αV (B,H) and β1b (C,I) morphants, DFCs did not aggregate properly, yielding a dysmorphic ZO-1 lattice (E,F). Anti-aPKCζ staining shows that KV cells in αV1miss morphants were polarized (G), but not in αV (H) and β1b morphants (I). Scale bars: 30 μm.

Expression Data

Gene:	EGFP
Antibodies:	Ab1-prkcz Ab1-tjp1 Ab1-tuba
Fish:	ha01Tg ha01Tg + MO1-itgav ha01Tg + MO3-itgb1b
Knockdown Reagents:	MO1-itgav MO3-itgb1b
Anatomical Terms:	Kupffer's vesicle Kupffer's vesicle apical junction complex Kupffer's vesicle bicellular tight junction Kupffer's vesicle cilium
Stage:	5-9 somites

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	ha01Tg + MO1-itgav ha01Tg + MO3-itgb1b
Knockdown Reagents:	MO1-itgav MO3-itgb1b
Observed In:	Kupffer's vesicle Kupffer's vesicle cilium Kupffer's vesicle epithelial cell Kupffer's vesicle development
Stage:	5-9 somites

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Development