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Fig. 8 KV lumen does not properly form in αV and β1b morphants. Confocal images of Tg(sox17:GFP)-expressing embryos (green) are shown. (A-C,G-I) Single focal planes at the center of the DFC cluster immunolabeled with anti-ZO-1 antibody (red; A-C) or anti-aPKC-ζ antibody (red) and anti-acetylated tubulin antibody (white; G-I). (D-F) 3D renderings of anti-ZO-1 labeled embryos of A-C. Dorsal views of 6-8 SS embryos are shown in all panels, anterior to the top. Embryos injected with αV1miss control MO developed a large fluid-filled lumen (A,G) that had a uniform ZO-1-labeled tight junction lattice within the DFC-derived lining of the KV (D). However, in αV (B,H) and β1b (C,I) morphants, DFCs did not aggregate properly, yielding a dysmorphic ZO-1 lattice (E,F). Anti-aPKCζ staining shows that KV cells in αV1miss morphants were polarized (G), but not in αV (H) and β1b morphants (I). Scale bars: 30 μm.