header logo image header logo text
Downloads Login
Research
General Information
ZIRC
ZFIN ID: ZDB-FIG-101021-52
Mueller et al., 2010 - Spatio-temporal regulation of Wnt and retinoic acid signaling by tbx16/spadetail during zebrafish mesoderm differentiation. BMC Genomics   11:492 Full text @ BMC Genomics
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Anatomical Term:
Stage Range: 1-4 somites to 5-9 somites
PHENOTYPE:
Fish:
Observed In:
Stage Range: 1-4 somites to 5-9 somites

Fig. 1 Expression of validated candidate genes from the 4/5-somite microarray. Changes in the expression of BI430182 (A, B, A′, B′), cebpa (C, D, C′, D′), drl (E, F, E′, F′), hmbsa (G, H, G′, H′), gtpbp1 (I, J, I′, J′) and calm1a (K, L, K′, L′) were visualized using in situ hybridization in wild-type (A, A′, C, C′, E, E′, G, G′, I, I′, K and K′) and spt embryos (B, B′, D, D′, F, F′, H, H′, J, J′, L and L′). All embryos are oriented with posterior to the right. Whole-mount embryos (A-L) are shown in lateral view while de-yolked and flat-mounted embryos (A′-L′) are shown in dorsal view. Flat-mounted embryos (A′-L′) show only the posterior half of the embryos for high-magnification view. All embryos were fixed at the 3 to 6-somite stage. Arrowheads point to specific intermediate mesoderm expression domain

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
calm1a WT standard conditions 1-4 somites to 5-9 somites intermediate mesoderm ISH
tbx16b104/b104 standard conditions 1-4 somites to 5-9 somites intermediate mesoderm ISH
cebpa WT standard conditions 1-4 somites to 5-9 somites intermediate mesoderm ISH
tbx16b104/b104 standard conditions 1-4 somites to 5-9 somites intermediate mesoderm ISH
drl WT standard conditions 1-4 somites to 5-9 somites intermediate mesoderm ISH
tbx16b104/b104 standard conditions 1-4 somites to 5-9 somites intermediate mesoderm ISH
gb:bi430182 WT standard conditions 1-4 somites to 5-9 somites intermediate mesoderm ISH
tbx16b104/b104 standard conditions 1-4 somites to 5-9 somites intermediate mesoderm ISH
gtpbp1 WT standard conditions 1-4 somites to 5-9 somites intermediate mesoderm ISH
tbx16b104/b104 standard conditions 1-4 somites to 5-9 somites intermediate mesoderm ISH
hmbsa WT standard conditions 1-4 somites to 5-9 somites intermediate mesoderm ISH
tbx16b104/b104 standard conditions 1-4 somites to 5-9 somites intermediate mesoderm ISH
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
tbx16b104/b104 standard conditions 1-4 somites - 5-9 somites intermediate mesoderm calm1a expression decreased amount, abnormal
1-4 somites - 5-9 somites intermediate mesoderm cebpa expression decreased amount, abnormal
1-4 somites - 5-9 somites intermediate mesoderm hmbsa expression decreased amount, abnormal
1-4 somites - 5-9 somites intermediate mesoderm gb:bi430182 expression decreased amount, abnormal
1-4 somites - 5-9 somites intermediate mesoderm gtpbp1 expression decreased amount, abnormal
1-4 somites - 5-9 somites intermediate mesoderm drl expression decreased amount, abnormal
Acknowledgments:
ZFIN wishes to thank the journal BMC Genomics for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ BMC Genomics