ZFIN ID: ZDB-FIG-100302-40
Millimaki et al., 2010 - Sox2 is required for maintenance and regeneration, but not initial development, of hair cells in the zebrafish inner ear. Developmental Biology   338(2):262-269 Full text @ Dev. Biol.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Conditions:
Knockdown Reagents:
Anatomical Terms:
Stage Range: 14-19 somites to Prim-25
PHENOTYPE:
Fish:
Condition:
Knockdown Reagent:
Observed In:
Stage: Prim-15

Fig. 3 Relationship between Sox2 and upstream regulators of hair cell development. (A–C) Expression of brn3c:gfp in hs:atoh1a/+ transgenic embryos (A and C) and a hs:atoh1a/+;hs:sox2/+ double transgenic embryo (B) heat shocked at 24 hpf and photographed at 34 hpf. The specimen in (C) was also injected with sox2-MO. The inset in (A) shows a heat-shocked brn3c:gfp/+ control embryo at 34 hpf. (D–L) sox2 expression in a control embryo (D), atoh1a/b morphants (E and F), wild-type embryos exposed to SU5402 (G), DAPT (H), or both DAPT and SU5402 (I) beginning at 26 hpf, a hs:atoh1a/+ embryo heat shocked at 24 hpf (J), a hs:gal4/+;UAS-NICD/+ embryo heat shocked at 24 hpf (K), and a hs:fgf8/+ embryo heat shocked at 30 hpf (L). sox2 expression is shown at 30 hpf, except (D) and (E) (18 hpf) and (L) (36 hpf). Expression in control embryos does not change appreciably between 30 and 36 hpf. All images show lateral views with anterior to the left and dorsal to the top.

Gene Expression Details
Gene Antibody Fish Conditions Stage Qualifier Anatomy Assay
GFP s356tTg; x20Tg heat shock Prim-15 hair cell IFL
Prim-15 otic vesicle hair cell IFL
s356tTg; x20Tg + MO2-sox2 heat shock Prim-15 hair cell IFL
Prim-15 otic vesicle hair cell IFL
s356tTg; x20Tg; x21Tg heat shock Prim-15 hair cell IFL
Prim-15 otic vesicle hair cell IFL
sox2 WT standard conditions 14-19 somites macula saccule ISH
14-19 somites macula utricle ISH
WT chemical treatment: DAPT Prim-15 macula saccule ISH
Prim-15 macula utricle ISH
WT chemical treatment: SU5402, chemical treatment: DAPT Prim-15 Not Detected macula saccule ISH
Prim-15 Not Detected macula utricle ISH
WT chemical treatment: SU5402 Prim-15 macula saccule ISH
Prim-15 macula utricle ISH
WT + MO1-atoh1b + MO4-atoh1a standard conditions 14-19 somites Not Detected macula saccule ISH
14-19 somites Not Detected macula utricle ISH
Prim-15 macula saccule ISH
Prim-15 macula utricle ISH
kca3Tg/+; kca4Tg/+ heat shock Prim-15 macula saccule ISH
Prim-15 macula utricle ISH
Prim-15 otic vesicle ISH
x17Tg heat shock Prim-25 macula saccule ISH
Prim-25 macula utricle ISH
Prim-25 otic vesicle ISH
x20Tg heat shock Prim-15 macula saccule ISH
Prim-15 macula utricle ISH
Prim-15 otic vesicle ISH
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
s356tTg; x20Tg heat shock Prim-15 optic vesicle hair cell displaced, abnormal
Prim-15 optic vesicle has extra parts of type hair cell, abnormal
s356tTg; x20Tg + MO2-sox2 heat shock Prim-15 optic vesicle has extra parts of type hair cell, abnormal
s356tTg; x20Tg; x21Tg heat shock Prim-15 optic vesicle hair cell disorganized, abnormal
Prim-15 optic vesicle hair cell displaced, abnormal
Acknowledgments:
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Reprinted from Developmental Biology, 338(2), Millimaki, B.B., Sweet, E.M., and Riley, B.B., Sox2 is required for maintenance and regeneration, but not initial development, of hair cells in the zebrafish inner ear, 262-269, Copyright (2010) with permission from Elsevier. Full text @ Dev. Biol.