ZFIN ID: ZDB-FIG-100302-38
Millimaki et al., 2010 - Sox2 is required for maintenance and regeneration, but not initial development, of hair cells in the zebrafish inner ear. Developmental Biology   338(2):262-269 Full text @ Dev. Biol.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Condition:
Knockdown Reagent:
Anatomical Terms:
Stage Range: 10-13 somites to Pec-fin
PHENOTYPE:
Fish:
Condition:
Observed In:
Stage Range: Prim-15 to Pec-fin

Fig. 1 Sox2 is not required for hair cell development. (A–C) sox2 expression in control embryos at 14 hpf (A), 30 hpf (B) and in a cross section of the utricular macula at 48 hpf (C). sox2 expression is lost from mature hair cells (hc) but is still detected in recently formed hair cells (arrows) and all surrounding support cells (arrowhead). (D) sox2 expression at 30 hpf in a sox2 morphant. (E–H) Expression of atoh1a in control embryos (E and G) and sox2 morphants (F and H) at the indicated times. Arrowheads mark macular expression domains. (I–P) brn3c:gfp expression in control embryos at 24 hpf (I), 48 hpf (J) and 60 hpf (K); expression in a control embryo heat shocked at 24 hpf and photographed at 40 hpf (L); expression in sox2 morphants at 24 hpf (M), 48 hpf (N) and 60 hpf (O); and expression in a hs:sox2 transgenic embryo heat shocked at 24 hpf and photographed at 40 hpf (P). Positions of the utricular (u) and saccular (s) maculae are indicated. Note the absence of hair cells in the middle of the saccular macula in the sox2 morphant (N). Arrows in (O) and (P) show hair cells being extruded from the utricular macula. All images show lateral views with anterior to the left and dorsal to the top. (Q) A time course showing the mean number of utricular hair cells in control embryos (con) and sox2 morphants (sox2 mo). Sox2 morphants exhibited a normal number of hair cells at 24 hpf (p = 0.88) but showed significantly fewer hair cells at later time points (p < 0.0001 for each time point). (R) Number of utricular hair cells in control embryos and hs:sox2/+ embryos subjected to heat shock at 18, 24 or 30 hpf, and counted at 40, 42 or 48 hpf, respectively. Transgenic embryos heat shocked at 18 hpf produced significantly more hair cells than normal (p < 0.0004), whereas the number of hair cells was not altered by heat shocking at 24 or 30 hpf (p = 0.78 or 0.73, respectively). Error bars in (Q) and (R) represent standard deviations, with n ≥ 15 for each time point.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
atoh1a WT standard conditions 10-13 somites otic placode ISH
Prim-15 macula saccule ISH
Prim-15 macula utricle ISH
WT + MO2-sox2 standard conditions 10-13 somites otic placode ISH
Prim-15 macula saccule ISH
Prim-15 macula utricle ISH
GFP s356tTg standard conditions Prim-5 hair cell IFL
Prim-5 hair cell anterior macula IFL
Prim-5 hair cell posterior macula IFL
Long-pec hair cell IFL
Long-pec hair cell anterior macula IFL
Long-pec hair cell posterior macula IFL
Pec-fin hair cell IFL
Pec-fin hair cell anterior macula IFL
s356tTg + MO2-sox2 standard conditions Prim-5 hair cell IFL
Prim-5 hair cell anterior macula IFL
Prim-5 hair cell posterior macula IFL
Long-pec hair cell IFL
Long-pec hair cell anterior macula IFL
Long-pec hair cell posterior macula IFL
Pec-fin hair cell IFL
Pec-fin hair cell anterior macula IFL
s356tTg; x21Tg standard conditions Prim-25 hair cell IFL
Prim-25 hair cell anterior macula IFL
s356tTg; x21Tg heat shock Prim-25 hair cell IFL
Prim-25 hair cell anterior macula IFL
sox2 WT standard conditions 10-13 somites macula saccule ISH
10-13 somites macula utricle ISH
10-13 somites otic placode ISH
Prim-15 macula saccule ISH
Prim-15 macula utricle ISH
Long-pec auditory epithelial support cell ISH
Long-pec immature hair cell anterior macula ISH
Long-pec immature hair cell posterior macula ISH
WT + MO2-sox2 standard conditions Prim-15 macula saccule ISH
Prim-15 macula utricle ISH
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
s356tTg standard conditions Prim-15 macula utricle has fewer parts of type hair cell, abnormal
Prim-25 macula utricle has fewer parts of type hair cell, abnormal
Long-pec inner ear receptor cell development increased duration, abnormal
Long-pec macula utricle has fewer parts of type hair cell, abnormal
Pec-fin hair cell anterior macula disorganized, abnormal
Pec-fin hair cell anterior macula protruding out of macula utricle, abnormal
Pec-fin inner ear receptor cell development increased duration, abnormal
s356tTg; x21Tg heat shock Prim-25 hair cell anterior macula protruding out of macula utricle, abnormal
Prim-25 macula utricle has extra parts of type hair cell, abnormal
Acknowledgments:
This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image.

Reprinted from Developmental Biology, 338(2), Millimaki, B.B., Sweet, E.M., and Riley, B.B., Sox2 is required for maintenance and regeneration, but not initial development, of hair cells in the zebrafish inner ear, 262-269, Copyright (2010) with permission from Elsevier. Full text @ Dev. Biol.