Community Action Needed: Please respond to the NIH RFI
ZFIN ID: ZDB-FIG-091221-21
Alexa et al., 2009 - Maternal and zygotic aldh1a2 activity is required for pancreas development in zebrafish. PLoS One   4(12):e8261 Full text @ PLoS One
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Condition:
Anatomical Terms:
Stage Range: Prim-5 to Prim-15

Fig. 3 aldh1a2um22 and aldh1a2i26 mutant embryos retain some endoderm gene expression at 24 and 30 hpf.

DMSO treated wild type embryos (A, E, I, M), DEAB-treated wild type embryos (B, F, J, N), embryos from an incross of aldh1a2um22 heterozygotes (C, G, K, O) and embryos from an incross of aldh1a2i26 heterozygotes (D, H, L, P) were assayed for expression of ins at 24 hpf (A–D; black arrows indicate residual expression), pdx1 at 24 hpf (E–H; black arrows indicate residual expression), hhex at 30 hpf (I–L; residual expression is indicated in pancreas (arrow) and liver (arrowhead)) and prox1 at 30 hpf (M–P; residual expression is indicated in liver (arrowhead)). Embryos are in dorsal view with anterior to the left. See Table 2 for quantification.

Gene Expression Details
Gene Antibody Fish Conditions Stage Qualifier Anatomy Assay
hhex WT control Prim-15 liver ISH
Prim-15 pancreatic bud ISH
WT chemical treatment: 4-(diethylamino)benzaldehyde Prim-15 Not Detected liver ISH
Prim-15 Not Detected pancreatic bud ISH
aldh1a2i26/i26 standard conditions Prim-15 liver ISH
Prim-15 pancreatic bud ISH
aldh1a2um22/um22 standard conditions Prim-15 liver ISH
Prim-15 pancreatic bud ISH
ins WT control Prim-5 posterior pancreatic bud ISH
WT chemical treatment: 4-(diethylamino)benzaldehyde Prim-5 Not Detected pancreatic bud ISH
aldh1a2i26/i26 standard conditions Prim-5 pancreatic bud ISH
aldh1a2um22/um22 standard conditions Prim-5 pancreatic bud ISH
pdx1 WT control Prim-5 pancreatic bud ISH
WT chemical treatment: 4-(diethylamino)benzaldehyde Prim-5 Not Detected pancreatic bud ISH
aldh1a2i26/i26 standard conditions Prim-5 pancreatic bud ISH
aldh1a2um22/um22 standard conditions Prim-5 pancreatic bud ISH
prox1a WT control Prim-15 liver ISH
Prim-15 pancreatic bud ISH
WT chemical treatment: 4-(diethylamino)benzaldehyde Prim-15 Not Detected liver ISH
Prim-15 Not Detected pancreatic bud ISH
aldh1a2i26/i26 standard conditions Prim-15 liver ISH
aldh1a2um22/um22 standard conditions Prim-15 liver ISH
Antibody Labeling Details No data available
Phenotype Details No data available
Acknowledgments:
ZFIN wishes to thank the journal PLoS One for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ PLoS One