ZFIN ID: ZDB-FIG-091221-20
Alexa et al., 2009 - Maternal and zygotic aldh1a2 activity is required for pancreas development in zebrafish. PLoS One   4(12):e8261 Full text @ PLoS One
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Condition:
Anatomical Terms:
Stage Range: Long-pec to Protruding-mouth

Fig. 4 aldh1a2um22 and aldh1a2i26 mutant embryos retain some endoderm gene expression at 48 and 72 hpf.

DMSO treated wild type embryos (A, E, I, M, Q), DEAB-treated wild type embryos (B, F, J, N, R), embryos from an incross of aldh1a2um22 heterozygotes (C, G, K, O, S) and embryos from an incross of aldh1a2i26 heterozygotes (D, H, L, P, T) were assayed for expression of pdx1 at 48 hpf (A–D), sid4 at 48 hpf (E–H), carbA at 72 hpf (I–L), ifabp at 72 hpf (M–P) and transf at 72 hpf (Q–T). Gene expression is observed in the intestine (open arrows), liver (black arrowheads) and pancreas (black arrows). Embryos are in dorsal view with anterior to the left. See Table 2 for quantification.

Gene Expression Details
Gene Antibody Fish Conditions Stage Qualifier Anatomy Assay
cpa5 WT control Protruding-mouth pancreas ISH
WT chemical treatment: 4-(diethylamino)benzaldehyde Protruding-mouth Not Detected pancreas ISH
aldh1a2i26/i26 standard conditions Protruding-mouth pancreas ISH
aldh1a2um22/um22 standard conditions Protruding-mouth Not Detected pancreas ISH
fabp2 WT control Protruding-mouth intestine ISH
WT chemical treatment: 4-(diethylamino)benzaldehyde Protruding-mouth Not Detected intestine ISH
aldh1a2i26/i26 standard conditions Protruding-mouth intestine ISH
aldh1a2um22/um22 standard conditions Protruding-mouth intestine ISH
pdx1 WT control Long-pec intestine ISH
Long-pec pancreas ISH
WT chemical treatment: 4-(diethylamino)benzaldehyde Long-pec Not Detected intestine ISH
Long-pec Not Detected pancreas ISH
aldh1a2i26/i26 standard conditions Long-pec intestine ISH
aldh1a2um22/um22 standard conditions Long-pec intestine ISH
sid4 WT control Long-pec liver ISH
WT chemical treatment: 4-(diethylamino)benzaldehyde Long-pec Not Detected liver ISH
aldh1a2i26/i26 standard conditions Long-pec liver ISH
aldh1a2um22/um22 standard conditions Long-pec liver ISH
tfa WT control Protruding-mouth liver ISH
WT chemical treatment: 4-(diethylamino)benzaldehyde Protruding-mouth Not Detected liver ISH
aldh1a2i26/i26 standard conditions Protruding-mouth liver ISH
aldh1a2um22/um22 standard conditions Protruding-mouth liver ISH
Antibody Labeling Details No data available
Phenotype Details No data available
Acknowledgments:
ZFIN wishes to thank the journal PLoS One for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ PLoS One