FIGURE

Fig. 5

ID

ZDB-FIG-091221-19

Publication

Alexa et al., 2009 - Maternal and zygotic aldh1a2 activity is required for pancreas development in zebrafish

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Fig. 5

aldh1a2 is maternally expressed and aldh1a2 translational morpholino knocks down endoderm expression.

A. PCR of 3 and 6 hpf wild type embryos using primers targeting exon1-2 and exon10-11 of aldh1a2 reveals aldh1a2 expression already at 3 hpf. A no DNA sample and amplification of tubulin is used as negative and positive controls. B?G. Wild type embryos were injected with either 950 uM aldh1a2 mismatch (mm) morpholino (MO; B, D, F) or 950 uM of aldh1a2 translational (tMO; C, E, G) and assayed for expression of hhex (B, C), prox1 (D, E) or pdx1 (F, G). Embryos are in dorsal view with anterior to the left.

Expression Data

Genes:	aldh1a2 hhex pdx1 prox1a
Fish:	WT WT + MO2-aldh1a2
Knockdown Reagent:	MO2-aldh1a2
Anatomical Terms:	intestine liver pancreas pancreatic bud whole organism
Stage Range:	1k-cell to Long-pec

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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