FIGURE

Fig. 3

ID

ZDB-FIG-091217-107

Publication

Hatler et al., 2009 - A gap junction connexin is required in the vertebrate left-right organizer

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Fig. 3

Molecular markers support a role for Cx43.4 in KV function. In situ hybridizations are used to probe for expression of genes in the L–R signaling cascade. (A–D) Twelve-somite Cx43.4 morphant embryos probed for spaw expression (arrowheads, throughout) show left, right, bilateral, and absent expression, respectively. spaw expression data are quantified (E) at 12 somites and (F) at 20 somites. Twenty-somite embryos probed with lefty1 show (G) normal expression in wild-type and (H) reversed expression in a DFC-targeted Cx43.4 knockdown embryo. (I) lefty1 data are summarized. (J) Twenty-somite embryos probed with lefty2 show a (K) reversal in the developing heart field of the Cx43.4 morphant. (L) Quantification of lefty2 expression was compiled from three independent experiments. Graphs in (E, F, I, L) represent means ± SEM from three independent experiments.

Expression Data

Genes:	lft1 lft2 spaw
Fish:	WT WT + MO1-gjc4b
Knockdown Reagent:	MO1-gjc4b
Anatomical Terms:	diencephalon left side diencephalon right side heart rudiment left side heart rudiment right side lateral mesoderm left side lateral mesoderm right side
Stage Range:	10-13 somites to 20-25 somites

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	WT + MO1-gjc4b
Knockdown Reagent:	MO1-gjc4b
Observed In:	determination of left/right symmetry
Stage Range:	10-13 somites to 20-25 somites

Phenotype Detail

Acknowledgments

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Reprinted from Developmental Biology, 336(2), Hatler, J.M., Essner, J.J., and Johnson, R.G., A gap junction connexin is required in the vertebrate left-right organizer, 183-191, Copyright (2009) with permission from Elsevier. Full text @ Dev. Biol.