FIGURE

Fig. 1

ID

ZDB-FIG-091217-105

Publication

Hatler et al., 2009 - A gap junction connexin is required in the vertebrate left-right organizer

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Fig. 1

Cx43.4 is specifically required for L–R patterning. Dorsal views of 30 hpf embryos processed for in situ hybridization with a cardiac myosin light chain probe show normal looping of the heart tube towards the right in controls (A) and aberrant looping to the left in Cx43.4 morphants (B). (C) Quantification of heart looping in four independent knockdown experiments is represented as means ± SEM. The dotted line in all graphs shows the theoretical maximum value for randomization. (D) Ventral view of autofluorescence in the gut of a 5 dpf larva, specifically the gall bladder (arrowhead) and pancreas (arrow); compare with (E) the Cx43.4 morphant where these organs are reversed.

Expression Data

Gene:	myl7
Fish:	WT WT + MO1-gjc4b
Knockdown Reagent:	MO1-gjc4b
Anatomical Term:	heart tube
Stage:	Prim-15

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	WT + MO1-gjc4b
Knockdown Reagent:	MO1-gjc4b
Observed In:	determination of digestive tract left/right asymmetry determination of heart left/right asymmetry gall bladder heart looping heart tube pancreatic bud
Stage Range:	Prim-15 to Day 5

Phenotype Detail

Acknowledgments

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Reprinted from Developmental Biology, 336(2), Hatler, J.M., Essner, J.J., and Johnson, R.G., A gap junction connexin is required in the vertebrate left-right organizer, 183-191, Copyright (2009) with permission from Elsevier. Full text @ Dev. Biol.