FIGURE

Fig. 2

ID

ZDB-FIG-091217-106

Publication

Hatler et al., 2009 - A gap junction connexin is required in the vertebrate left-right organizer

Other Figures

All Figure Page

Back to All Figure Page

Fig. 2

Cx43.4 is expressed in a spatiotemporal manner to be competent for KV function. Confocal fluorescence imaging (A) shows Cx43.4 labeling (red) in puncta that are distinct from the nuclei and cilia (green) of KV cells (arrow). Note also significant Cx43.4 labeling in the surrounding tail bud tissue. Grid-like confocal imaging (B) demonstrates Cx43.4 MO efficacy by the lack of staining (red), punctate or otherwise, in knockdown embryos. Cilia (green) of KV are labeled with tubulin antibodies and marked with an arrow as in (A). (C) Defects in heart laterality are partially rescued by coinjection of cx43.4 mRNA, ∗p = 0.04. (D) DFC-targeted injections of Cx43.4 MOs have a significant heart reversal phenotype. (C, D) Data are averages of three independent experiments and are represented as means ± SEM.

Expression Data

Gene:	gjc4b
Antibody:	Ab1-tuba
Fish:	WT WT + MO1-gjc4b
Knockdown Reagent:	MO1-gjc4b
Anatomical Terms:	Kupffer's vesicle Kupffer's vesicle cilium
Stage:	10-13 somites

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	WT + MO1-gjc4b WT + MO1-tbxta
Knockdown Reagents:	MO1-gjc4b MO1-tbxta
Observed In:	determination of heart left/right asymmetry heart looping heart tube
Stage:	Prim-15

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image.

Reprinted from Developmental Biology, 336(2), Hatler, J.M., Essner, J.J., and Johnson, R.G., A gap junction connexin is required in the vertebrate left-right organizer, 183-191, Copyright (2009) with permission from Elsevier. Full text @ Dev. Biol.