ZFIN ID: ZDB-FIG-090904-42
Jing et al., 2009 - Zebrafish ale oko, an essential determinant of sensory neuron survival and the polarity of retinal radial glia, encodes the p50 subunit of dynactin. Development (Cambridge, England)   136(17):2955-2964 Full text @ Development
Knockdown Reagents:
Observed In:
Stage: Day 4

Fig. 3 The ale oko locus encodes a zebrafish dynactin 2 homolog. (A) Map of the ako genomic region and exon/intron structure of the ako transcript. The position of mutant sequence in the akojj50 allele is indicated (red arrowhead). (B,C) Comparison of sequence trace data from wild-type (B) and mutant (C) animals. The akojj50 mutation activates a cryptic splice site in the genomic sequence. This, in turn, introduces a 9-bp insertion (red) that encodes a premature stop codon. (D) RT-PCR amplification of the dynactin 2 transcript using a primer that recognizes the mutant insertion sequence. Lane 1, DNA ladder; lane 2, amplification of the mutant transcript produces a single robust band; lane 3, amplification of the wild-type transcript does not produce a detectable product; lane 4, BfaI digestion confirms the identity of the amplification product from the mutant; lanes 5 and 6, control PCR amplification of actin from wild-type (5) and mutant (6) animals. The positions of amplification primers in the wild type and mutant are indicated on the diagram below. (E-H) Phenocopy and rescue of photoreceptor defects in ako mutant animals. Transverse cryosections through embryonic retinae were stained with the Zpr-1 antibody to visualize double cones (red). Green signal in E,F originates from the Tg(brn3c:mGFP) transgene. The injection of ako (H), but not GFP (G), mRNA rescues photoreceptor morphology in ako mutants. L, lens. Scale bar: 40 μm.

Gene Expression Details No data available
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
s356tTg + MO1-dctn2 + MO2-dctn2 standard conditions Day 4 eye decreased size, abnormal
Day 4 photoreceptor cell morphology, abnormal
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