ZFIN ID: ZDB-FIG-090904-40
Jing et al., 2009 - Zebrafish ale oko, an essential determinant of sensory neuron survival and the polarity of retinal radial glia, encodes the p50 subunit of dynactin. Development (Cambridge, England)   136(17):2955-2964 Full text @ Development
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Gene:
Antibodies:
Fish:
Anatomical Terms:
Stage Range: Pec-fin to Day 4
PHENOTYPE:
Fish:
Observed In:
Stage Range: Pec-fin to Day 5

Fig. 1 ale oko phenotype in the zebrafish retina. (A-B′) External phenotypes of akojj50 mutants (A′,B′), compared with their wild-type siblings (A,B). Dorsal (A,A′) and lateral (B,B′) views are shown at 5 dpf. (C-D′) Transverse sections of the ako (C′,D′) and wild-type (C,D) retinae at 4 dpf. (E,E′) Electron micrographs of wild-type (E) and mutant (E′) retinae at 3 dpf. OS, outer segment; IS, inner segment. Asterisks indicate abnormal mutant outer segments. (F-K′) Differentiation of retinal cells classes in ako mutants. Transverse cryosections through retinae of wild type (top) and akojj50 mutants (bottom). (F-G) Müller glia are visualized via the expression of a GFP transgene (F,F′, green, arrows) or by antibody staining against carbonic anhydrase (G,G′, red). Photoreceptors are stained with the Zpr-1 antibody (red in F,F′, green in G,G′). (H-J′) Transverse sections stained with antibodies to subpopulations of amacrine cells: anti-serotonin (H,H′, green), anti-neuropeptide Y (I,I′, red, arrows), anti-parvalbumin (I,I′, green) and anti-GABA (J,J′). (K,K) Sections through retinae of Tg(brn3c:mGFP) transgenic animals, which express GFP in ganglion cells (green). Photoreceptors are visualized with the Zpr-1 antibody (red). In G-K′, asterisks indicate the optic nerve. L, lens; rpe, retinal pigment epithelium; prcl, photoreceptor cell layer; inl, inner nuclear layer; ipl, inner plexiform layer; gcl, ganglion cell layer. Scale bars: 1 mm in A-B′; 40 μm in C,C′,F-K′; 30 μm in D,D′; 5 μm in E,E′.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
GFP dctn2jj50/jj50; mi2001Tg standard conditions Pec-fin Muller cell IFL
dctn2jj50/jj50; s356tTg standard conditions Day 4 retinal ganglion cell IFL
mi2001Tg standard conditions Pec-fin Muller cell IFL
s356tTg standard conditions Day 4 retinal ganglion cell IFL
Antibody Labeling Details
Antibody Assay Fish Conditions Stage Anatomy
Ab1-ca IHC WT standard conditions Day 4 Muller cell
IHC dctn2jj50/jj50 (AB) standard conditions Day 4 Muller cell
Ab1-GABA IHC WT standard conditions Day 4 amacrine cell
IHC dctn2jj50/jj50 (AB) standard conditions Day 4 amacrine cell
Ab1-npy IHC WT standard conditions Day 4 amacrine cell
IHC dctn2jj50/jj50 (AB) standard conditions Day 4 amacrine cell
zpr-1 IHC WT standard conditions Day 4 photoreceptor cell
IHC dctn2jj50/jj50 (AB) standard conditions Day 4 photoreceptor cell
IHC dctn2jj50/jj50; mi2001Tg standard conditions Pec-fin photoreceptor cell
IHC dctn2jj50/jj50; s356tTg standard conditions Day 4 photoreceptor cell
IHC mi2001Tg standard conditions Pec-fin photoreceptor cell
IHC s356tTg standard conditions Day 4 photoreceptor cell
Phenotype Details
Fish Conditions Stage Phenotype
dctn2jj50/jj50 (AB) standard conditions Protruding-mouth photoreceptor cell morphology, abnormal
Protruding-mouth photoreceptor outer segment layer photoreceptor outer segment decreased amount, abnormal
Protruding-mouth photoreceptor outer segment layer photoreceptor outer segment deformed, abnormal
Day 4 photoreceptor cell decreased amount, abnormal
Day 4 photoreceptor cell morphology, abnormal
Day 4 photoreceptor outer segment layer photoreceptor outer segment decreased amount, abnormal
Day 4 photoreceptor outer segment layer photoreceptor outer segment deformed, abnormal
Day 5 eye decreased size, abnormal
dctn2jj50/jj50; mi2001Tg standard conditions Pec-fin Muller cell decreased amount, abnormal
Acknowledgments:
This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Development