ZFIN ID: ZDB-FIG-090904-41
Jing et al., 2009 - Zebrafish ale oko, an essential determinant of sensory neuron survival and the polarity of retinal radial glia, encodes the p50 subunit of dynactin. Development (Cambridge, England)   136(17):2955-2964 Full text @ Development
Observed In:
Stage: Day 5

Fig. 2 ale oko hair cell phenotype. (A-B′) Whole-mount staining of ear sensory maculae with anti-acetylated-α-tubulin antibody (green) and phalloidin (red, actin) to visualize hair cells and their stereocilia, respectively. Posterior (A′) and anterior (B′) maculae of ako mutants are shown. (C,C′) Electron micrographs of auditory hair cells in the posterior macula of wild-type (C) and ako mutant (C′) zebrafish larvae. Cellular debris (arrowheads) is present in the mutant tissue. (D,D′) In the ako mutant lateral line (D′), few hair cells survive by 5 dpf compared with the wild type (D). (E) Quantitation of hair cell numbers in the maculae of wild-type and akojj50 animals at 5 dpf. The average numbers of hair cells in the anterior (AM) and the posterior (PM) maculae are provided (n=6). For posterior macula, P<0.01 (Student′s t-test). (F) The average number of hair cells per lateral line neuromast in wild-type and akojj50 animals (n=6). At 4 and 5 dpf, P<0.0001 (Student′s t-test). A,A′,D,D′ show face views of the apical surface; in B-C′, apical is up. Scale bars: 5 μm in C,C′; 20 μm in A-B′,D,D′.

Gene Expression Details No data available
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
dctn2jj50/jj50 (AB) standard conditions Day 5 hair cell posterior macula decreased amount, abnormal
dctn2jj50/jj50; s356tTg standard conditions Day 5 neuromast hair cell decreased amount, abnormal
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