FIGURE

Fig. 2

ID

ZDB-FIG-090804-2

Publication

Kurrasch et al., 2009 - Neuroendocrine transcriptional programs adapt dynamically to the supply and demand for neuropeptides as revealed in NSF mutant zebrafish

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Fig. 2

Loss of hypothalamic markers occurs during embryo-to-larva transition. (A) Cartoon depicting the spatial patterning of known hypothalamic markers in the zebrafish (48 hpf) and mouse (P0) brain. A dorsal view, ventral side down is shown for zebrafish and a horizontal plane is shown for mouse of several conserved hypothalamic transcription factors and neuropeptides. Other anatomical landmarks include the eyes, yolk sac, third ventricle (3V), and on the mouse cartoon, amygdala (A), (B) Whole mount expression patterns are shown for nkx2.1a, nr5a (ff1d), hcrt, avpl, and crh for both 48-hpf (n = 15) and 5-dpf (n = 20) wild-type (WT) and nsf^st53zebrafish. The expression of hypothalamic markers is lost by 5 dpf (right panel). The 48-hpf nsf^st53zebrafish were identified by genotyping. (C) Relative transcript levels of neuroendocrine markers are shown in wild-type and nsf^st53zebrafish heads at 5 dpf (n = 8 each). Fold induction is relative to 1.0 with results from a single wild-type head chosen for each primer set. Samples variation is shown by error bars (standard error of the mean).

Expression Data

Genes:	avp crhb hcrt nkx2.4b nr5a1b
Fish:	WT nsfa^st53/st53
Anatomical Term:	hypothalamus
Stage Range:	Long-pec to Day 5

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	nsfa^st53/st53
Observed In:	hypothalamus development
Stage:	Day 5

Phenotype Detail

Acknowledgments

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