FIGURE

Fig. 4

ID

ZDB-FIG-090804-4

Publication

Kurrasch et al., 2009 - Neuroendocrine transcriptional programs adapt dynamically to the supply and demand for neuropeptides as revealed in NSF mutant zebrafish

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Fig. 4

nsf^st53hypothalamus undergoes normal development and does not appear to initiate apoptosis. Apoptosis in (A) wild-type (WT) and (B) nsf^st535 dpf zebrafish (12 μm horizontal sections) is shown. Cell death is not observed in the hypothalamus (B), but is observed in olfactory terminals (B, white arrowhead). Photoreceptor auto-fluorescence in wild-type zebrafish is lost in mutant (B, white arrow). DAPI staining of wild-type (A, C, E) and nsf^st53zebrafish (B, D, F) at 5 dpf is shown. Immunocytochemistry for (C, D) Hu family proteins and (E, F) SV2 are shown for wild-type and nsf^st535 dpf zebrafish. All images are representative of at least 15 animals assayed. (G-I) Electron microscopy images from nsf^st53hypothalamic sections (n = 3). Cell bodies (G) and vesicle-filled synapse (H) resemble wild-type neurons (data not shown). Anatomical landmarks and (I) the presence of dark-cored secretory vesicles (red arrowhead) were used to confirm the location of the hypothalamus. N = nucleus. Note that the medially located cell bodies (Figure 4C, D, G) are framed on either side by lateral projections (Figure 4E, F, H) in both cryosection and EM images.

Expression Data

Antibody:	Ab-SV2
Fish:	WT nsfa^st53/st53
Anatomical Term:	hypothalamus
Stage:	Day 5

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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