FIGURE

Fig. 10

ID

ZDB-FIG-090617-39

Publication

Bae et al., 2009 - Anatomy of zebrafish cerebellum and screen for mutations affecting its development

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Fig. 10

Presynaptic terminals of granule neurons (parallel fibers) and GABAergic interneurons. (A) Schematic presentation. (B) Immunoblotting of brain lysates with the anti-Vglut1 antibody. (C–E) Co-staining with anti-Pvalb7 (green) and anti-Vglut1 (magenta) antibodies. (C–E) Sagittal sections with anterior to the left; low (C), medium (D), and high (E) magnification views. (D, E) Corpus cerebelli (CCe). Vglut1⁺ dots (presynaptic boutons of parallel fibers) abutted the dendrites but not the soma (asterisk) of Purkinje cells in the ML. (F, G) Co-staining with anti-Pvalb7 (green) and anti-Gad1 (magenta) antibodies. Gad1⁺ dots (presynaptic boutons of GABAergic interneurons) abut the dendrites and somata (asterisks) of Purkinje cells. The abbreviations are as described in Fig. 1. Scale bars: 200 μm (C), 100 μm (F) and 20 μm (D, E, G).

Expression Data

Genes:	gad1b pvalb7 slc17a7a
Antibodies:	Ab1-gad1 Ab1-slc17a7
Fish:	WT
Anatomical Terms:	brain granular layer corpus cerebelli Golgi cell granular layer corpus cerebelli presynaptic active zone granule cell axon granule cell presynaptic active zone molecular layer corpus cerebelli molecular layer corpus cerebelli presynaptic active zone molecular layer corpus cerebelli stellate interneuron Purkinje cell dendrite Purkinje cell neuronal cell body
Stage:	Adult

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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Reprinted from Developmental Biology, 330(2), Bae, Y.K., Kani, S., Shimizu, T., Tanabe, K., Nojima, H., Kimura, Y., Higashijima, S.I., and Hibi, M., Anatomy of zebrafish cerebellum and screen for mutations affecting its development, 406-426, Copyright (2009) with permission from Elsevier. Full text @ Dev. Biol.