ZFIN ID: ZDB-FIG-090617-20
Park et al., 2009 - Isolation of a ventricle-specific promoter for the zebrafish ventricular myosin heavy chain (vmhc) gene and its regulation by GATA factors during embryonic heart development. Developmental dynamics : an official publication of the American Association of Anatomists   238(6):1574-1581 Full text @ Dev. Dyn.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Gene:
Fish:
Knockdown Reagents:
Anatomical Term:
Stage: Long-pec
PHENOTYPE:
Fish:
Knockdown Reagents:
Observed In:
Stage: Long-pec

Fig. 6 Antisense morpholino nucleotides (MOs) effectively affected development of heart as well as expression of vmhc promoter reporter gene (green fluorescent protein [GFP]). Living embryos are shown at 48 hours postfertilization (hpf). A-H: Representative transgenic embryos showing the effects of ventricle-specific GFP expression in vmhc:gfp compared with the control (A; Mock, injection buffer injected), GATA4 MO-injected (B; MO4), GATA5 MO-injected (C; MO5), GATA6 MO-injected (D; MO6), GATA4 and GATA5 MO-injected (E; MO4+5), GATA4 and GATA6 MO-injected (F; MO4+6), GATA5 and GATA6 MO-injected (G; MO5+6), GATA4, GATA5 and GATA6 MO-injected (H; MO4+5+6) embryos. At 48 hpf, the control embryos showed no significant difference in GFP expression compared with either the GATA4 or GATA6 morphants. GATA5 morphant displayed cardia bifida but the GFP intensity was not reduced (C). In contrast, simultaneous injection of GATA4 and GATA6 MOs resulted in embryos with significantly decreased ventricular GFP expression (F). Views are dorsal and anterior to the left. The percentages of embryo with each intensity are indicated in parentheses.

Gene Expression Details
Gene Antibody Fish Conditions Stage Qualifier Anatomy Assay
EGFP knu9Tg (AB) control Long-pec cardiac ventricle IFL
knu9Tg + MO1-gata5 (AB) standard conditions Long-pec cardiac ventricle IFL
knu9Tg + MO1-gata5 + MO1-gata6 (AB) standard conditions Long-pec cardiac ventricle IFL
knu9Tg + MO1-gata5 + MO1-gata6 + MO5-gata4 (AB) standard conditions Long-pec Not Detected whole organism IFL
knu9Tg + MO1-gata5 + MO5-gata4 (AB) standard conditions Long-pec cardiac ventricle IFL
knu9Tg + MO1-gata6 (AB) standard conditions Long-pec cardiac ventricle IFL
knu9Tg + MO1-gata6 + MO5-gata4 (AB) standard conditions Long-pec cardiac ventricle IFL
knu9Tg + MO5-gata4 (AB) standard conditions Long-pec cardiac ventricle IFL
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
knu9Tg + MO1-gata5 (AB) standard conditions Long-pec heart bifurcated, abnormal
Long-pec heart morphogenesis process quality, abnormal
knu9Tg + MO1-gata5 + MO1-gata6 + MO5-gata4 (AB) standard conditions Long-pec heart absent, abnormal
Long-pec whole organism EGFP expression absent, abnormal
knu9Tg + MO1-gata6 + MO5-gata4 (AB) standard conditions Long-pec cardiac ventricle EGFP expression decreased distribution, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Developmental dynamics : an official publication of the American Association of Anatomists for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Dev. Dyn.