PUBLICATION

Isolation of a ventricle-specific promoter for the zebrafish ventricular myosin heavy chain (vmhc) gene and its regulation by GATA factors during embryonic heart development

Authors
Park, J.S., Kim, H.S., Kim, J.D., Seo, J., Chung, K.S., Lee, H.S., Huh, T.L., Jo, I., and Kim, Y.O.
ID
ZDB-PUB-090518-20
Date
2009
Source
Developmental dynamics : an official publication of the American Association of Anatomists   238(6): 1574-1581 (Journal)
Registered Authors
Huh, Tae-Lin, Kim, Hyung-Seok, Kim, Jun-Dae
Keywords
Myh-15, β-MHC, GATA4, GATA6, transgenic, cardiac, myocardium
MeSH Terms
  • Animals
  • Animals, Genetically Modified
  • Base Sequence
  • GATA Transcription Factors/genetics
  • GATA Transcription Factors/metabolism*
  • Gene Expression Regulation, Developmental*
  • Genes, Reporter
  • Heart Ventricles*/embryology
  • Heart Ventricles*/metabolism
  • Molecular Sequence Data
  • Myosin Heavy Chains*/genetics
  • Myosin Heavy Chains*/metabolism
  • Oligonucleotides, Antisense/genetics
  • Oligonucleotides, Antisense/metabolism
  • Promoter Regions, Genetic*
  • Recombinant Fusion Proteins/genetics
  • Recombinant Fusion Proteins/metabolism
  • Zebrafish*/anatomy & histology
  • Zebrafish*/embryology
  • Zebrafish*/genetics
  • Zebrafish Proteins/genetics
  • Zebrafish Proteins/metabolism*
PubMed
19441085 Full text @ Dev. Dyn.
Abstract
We investigated chamber-specific gene expression by isolating a 2.2-kb polymerase chain reaction product containing the 5'-flanking region of the zebrafish ventricular myosin heavy-chain gene (vmhc). Promoter analysis revealed that the fragment, consisting of nucleotides from -301 to +26, is sufficient for vmhc promoter activity. Among several putative cis-acting elements in the region, a GATA-binding site was identified to be crucial for the activity of the promoter, as evidenced by the complete abolishment of promoter activity by a single nucleotide substitution of GATA-binding site (-287, C-->T). Knockdown of GATA-binding proteins 4 and 6 (GATA4 and -6) by their antisense morpholino oligonucleotides resulted in reduced green fluorescent protein (GFP) reporter gene and endogenous vmhc expression. These findings suggest that GATA4 and -6 play a key role in the regulation of vmhc expression in the ventricle. In addition, the vmhc promoter and the transgenic zebrafish (vmhc:gfp) are useful tools to study the formation and function of the ventricle.
Genes / Markers
Figures
Figure Gallery
Expression
Phenotype
Mutation and Transgenics
Human Disease / Model Data
Sequence Targeting Reagents
Fish
Antibodies
Orthology
Engineered Foreign Genes
Errata and Notes