PUBLICATION
Isolation of a ventricle-specific promoter for the zebrafish ventricular myosin heavy chain (vmhc) gene and its regulation by GATA factors during embryonic heart development
- Authors
- Park, J.S., Kim, H.S., Kim, J.D., Seo, J., Chung, K.S., Lee, H.S., Huh, T.L., Jo, I., and Kim, Y.O.
- ID
- ZDB-PUB-090518-20
- Date
- 2009
- Source
- Developmental Dynamics : an official publication of the American Association of Anatomists 238(6): 1574-1581 (Journal)
- Registered Authors
- Huh, Tae-Lin, Kim, Hyung-Seok, Kim, Jun-Dae
- Keywords
- Myh-15, β-MHC, GATA4, GATA6, transgenic, cardiac, myocardium
- MeSH Terms
-
- Animals
- Animals, Genetically Modified
- Base Sequence
- GATA Transcription Factors/genetics
- GATA Transcription Factors/metabolism*
- Gene Expression Regulation, Developmental*
- Genes, Reporter
- Heart Ventricles*/embryology
- Heart Ventricles*/metabolism
- Molecular Sequence Data
- Myosin Heavy Chains*/genetics
- Myosin Heavy Chains*/metabolism
- Oligonucleotides, Antisense/genetics
- Oligonucleotides, Antisense/metabolism
- Promoter Regions, Genetic*
- Recombinant Fusion Proteins/genetics
- Recombinant Fusion Proteins/metabolism
- Zebrafish*/anatomy & histology
- Zebrafish*/embryology
- Zebrafish*/genetics
- Zebrafish Proteins/genetics
- Zebrafish Proteins/metabolism*
- PubMed
- 19441085 Full text @ Dev. Dyn.
Citation
Park, J.S., Kim, H.S., Kim, J.D., Seo, J., Chung, K.S., Lee, H.S., Huh, T.L., Jo, I., and Kim, Y.O. (2009) Isolation of a ventricle-specific promoter for the zebrafish ventricular myosin heavy chain (vmhc) gene and its regulation by GATA factors during embryonic heart development. Developmental Dynamics : an official publication of the American Association of Anatomists. 238(6):1574-1581.
Abstract
We investigated chamber-specific gene expression by isolating a 2.2-kb polymerase chain reaction product containing the 5'-flanking region of the zebrafish ventricular myosin heavy-chain gene (vmhc). Promoter analysis revealed that the fragment, consisting of nucleotides from -301 to +26, is sufficient for vmhc promoter activity. Among several putative cis-acting elements in the region, a GATA-binding site was identified to be crucial for the activity of the promoter, as evidenced by the complete abolishment of promoter activity by a single nucleotide substitution of GATA-binding site (-287, C-->T). Knockdown of GATA-binding proteins 4 and 6 (GATA4 and -6) by their antisense morpholino oligonucleotides resulted in reduced green fluorescent protein (GFP) reporter gene and endogenous vmhc expression. These findings suggest that GATA4 and -6 play a key role in the regulation of vmhc expression in the ventricle. In addition, the vmhc promoter and the transgenic zebrafish (vmhc:gfp) are useful tools to study the formation and function of the ventricle.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping