FIGURE
Fig. 5
- ID
- ZDB-FIG-090617-19
- Publication
- Park et al., 2009 - Isolation of a ventricle-specific promoter for the zebrafish ventricular myosin heavy chain (vmhc) gene and its regulation by GATA factors during embryonic heart development
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Fig. 5
GATA-binding site is essential for ventricle-specific vmhc expression. A: The regulatory region (-301/-277) of vmhc contains two transcription factor-binding sites, Smad and GATA (boxes). B: Zebrafish (3 days postfertilization, dpf) injected with plasmid vmhc-301 showed transient expression of green fluorescent protein (GFP). C: When GTC at -300 of vmhc-301 (Smad-binding site) was replaced by CAT, the GFP reporter gene was still expressed in the ventricle with a slight reduction of intensity. D: A single nucleotide substitution of the GATA site (C→T at -287) completely abolished GFP expression. |
Expression Data
Expression Detail
Antibody Labeling
Phenotype Data
Phenotype Detail
Acknowledgments
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