ZFIN ID: ZDB-FIG-090617-16
Park et al., 2009 - Isolation of a ventricle-specific promoter for the zebrafish ventricular myosin heavy chain (vmhc) gene and its regulation by GATA factors during embryonic heart development. Developmental Dynamics : an official publication of the American Association of Anatomists   238(6):1574-1581 Full text @ Dev. Dyn.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Gene:
Fish:
Anatomical Terms:
Stage Range: 20-25 somites to Prim-5
PHENOTYPE:
Fish:
Knockdown Reagents:
Observed In:
Stage: Long-pec

Fig. 2 Identical expression pattern of green fluorescent protein (GFP) reporter gene by vmhc promoter to endogenous vmhc mRNA in the heart region at the early heart-forming stage. A,C,E: Expression patterns of endogenous vmhc mRNA are shown in heart region at 18, 21.5, 24 hours postfertilization (hpf)each. Dorsal views with anterior to the left. B,D,F: Endogenous vmhc transcripts are observed in trunk muscle. Lateral view with anterior to the left. G,I,K: Expression patterns of gfp mRNA are observed in the heart region. Dorsal views with anterior to the left. H,J,L: No gfp transcript is observed in the trunk region. Lateral view with anterior to the left. M,N: GFP fluorescence signals from transgenic zebrafish embryos in heart region at 21.5 and 24 hpf. Dorsal views with anterior to the left. h, heart; tm, trunk muscle.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
EGFP knu9Tg (AB) standard conditions 20-25 somites heart rudiment IFL
Prim-5 heart tube IFL
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
knu9Tg + MO1-gata6 (AB) standard conditions Long-pec heart morphogenesis process quality, abnormal
knu9Tg + MO5-gata4 (AB) standard conditions Long-pec heart morphogenesis process quality, abnormal
Acknowledgments:
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