|ZFIN ID: ZDB-FIG-090617-16|
|Stage Range:||20-25 somites to Prim-5|
Fig. 2 Identical expression pattern of green fluorescent protein (GFP) reporter gene by vmhc promoter to endogenous vmhc mRNA in the heart region at the early heart-forming stage. A,C,E: Expression patterns of endogenous vmhc mRNA are shown in heart region at 18, 21.5, 24 hours postfertilization (hpf)each. Dorsal views with anterior to the left. B,D,F: Endogenous vmhc transcripts are observed in trunk muscle. Lateral view with anterior to the left. G,I,K: Expression patterns of gfp mRNA are observed in the heart region. Dorsal views with anterior to the left. H,J,L: No gfp transcript is observed in the trunk region. Lateral view with anterior to the left. M,N: GFP fluorescence signals from transgenic zebrafish embryos in heart region at 21.5 and 24 hpf. Dorsal views with anterior to the left. h, heart; tm, trunk muscle.
|EGFP||knu9Tg(AB)||standard conditions||20-25 somites||heart rudiment||IFL|
|knu9Tg + MO1-gata6(AB)||standard conditions||Long-pec||heart morphogenesis process quality, abnormal|
|knu9Tg + MO5-gata4(AB)||standard conditions||Long-pec||heart morphogenesis process quality, abnormal|
|Acknowledgments:||ZFIN wishes to thank the journal Developmental dynamics : an official publication of the American Association of Anatomists for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Dev. Dyn.|