ZFIN Figure: Park et al., 2009, Fig. 2

ZFIN ID: ZDB-FIG-090617-16

ZFIN ID: ZDB-FIG-090617-16

Park et al., 2009 - Isolation of a ventricle-specific promoter for the zebrafish ventricular myosin heavy chain (vmhc) gene and its regulation by GATA factors during embryonic heart development. Developmental Dynamics : an official publication of the American Association of Anatomists 238(6):1574-1581 Full text @ Dev. Dyn.

ADDITIONAL FIGURES

EXPRESSION / LABELING:

Gene:	EGFP
Fish:	knu9Tg (AB)
Anatomical Terms:	heart rudiment heart tube
Stage Range:	20-25 somites to Prim-5

PHENOTYPE:

Fish:	knu9Tg + MO1-gata6 (AB) knu9Tg + MO5-gata4 (AB)
Knockdown Reagents:	MO1-gata6 MO5-gata4
Observed In:	heart morphogenesis
Stage:	Long-pec

Fig. 2 Identical expression pattern of green fluorescent protein (GFP) reporter gene by vmhc promoter to endogenous vmhc mRNA in the heart region at the early heart-forming stage. A,C,E: Expression patterns of endogenous vmhc mRNA are shown in heart region at 18, 21.5, 24 hours postfertilization (hpf)each. Dorsal views with anterior to the left. B,D,F: Endogenous vmhc transcripts are observed in trunk muscle. Lateral view with anterior to the left. G,I,K: Expression patterns of gfp mRNA are observed in the heart region. Dorsal views with anterior to the left. H,J,L: No gfp transcript is observed in the trunk region. Lateral view with anterior to the left. M,N: GFP fluorescence signals from transgenic zebrafish embryos in heart region at 21.5 and 24 hpf. Dorsal views with anterior to the left. h, heart; tm, trunk muscle.

Gene Expression Details

Gene	Antibody	Fish	Conditions	Stage	Anatomy	Assay
EGFP		knu9Tg (AB)	standard conditions	20-25 somites	heart rudiment	IFL
				Prim-5	heart tube	IFL

Antibody Labeling Details No data available

Phenotype Details

Fish	Conditions	Stage	Phenotype
knu9Tg + MO1-gata6 (AB)	standard conditions	Long-pec	heart morphogenesis process quality, abnormal
knu9Tg + MO5-gata4 (AB)	standard conditions	Long-pec	heart morphogenesis process quality, abnormal

Acknowledgments:
This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Dev. Dyn.