FIGURE

Fig. 3

ID

ZDB-FIG-090506-47

Publication

Alvarez-Delfin et al., 2009 - Tbx2b is required for ultraviolet photoreceptor cell specification during zebrafish retinal development

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Fig. 3

Increased rod number and fewer UV cones in lor^p25bbtl and fby mutants. (A–C) Confocal images of eyes from 5-dpf WT (A), lor^p25bbtl (B), and fby (C) larvae, immunolabeled for UV opsin (green) and rods (red). Eyes from lor^p25bbtl and fby mutant larvae demonstrate a dramatic deficit in UV cones, with fby displaying a more severe phenotype; in this particular sample, no UV cones were detected. (D) Graph showing the average number of rods (red) and UV cones (green) per unit area quantified from confocal images of lor^p25bbtl mutant and WT retinas (mean + SD). (E and F) Expression analysis of opsins (E) and arrestins (F) by RT-PCR from RNA extracted from 5-dpf WT and lor^p25bbtl larvae. The RT-PCR product for UV opsin was reduced and products for rod opsin and rod arrestin were increased in lor^p25bbtl (red boxes).

Expression Data

Genes:	opn1sw1 opn1sw2 rho
Antibody:	Ab-4C12
Fish:	WT tbx2b^{p25bbtl/p25bbtl}
Anatomical Terms:	retinal rod cell whole organism
Stage:	Day 5

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	tbx2b^c144/c144 tbx2b^{p25bbtl/p25bbtl}
Observed In:	retinal cone cell retinal rod cell
Stage:	Day 5

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Proc. Natl. Acad. Sci. USA