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Fig. 3 Increased rod number and fewer UV cones in lorp25bbtl and fby mutants. (A–C) Confocal images of eyes from 5-dpf WT (A), lorp25bbtl (B), and fby (C) larvae, immunolabeled for UV opsin (green) and rods (red). Eyes from lorp25bbtl and fby mutant larvae demonstrate a dramatic deficit in UV cones, with fby displaying a more severe phenotype; in this particular sample, no UV cones were detected. (D) Graph showing the average number of rods (red) and UV cones (green) per unit area quantified from confocal images of lorp25bbtl mutant and WT retinas (mean + SD). (E and F) Expression analysis of opsins (E) and arrestins (F) by RT-PCR from RNA extracted from 5-dpf WT and lorp25bbtl larvae. The RT-PCR product for UV opsin was reduced and products for rod opsin and rod arrestin were increased in lorp25bbtl (red boxes).