ZFIN ID: ZDB-FIG-090501-55
Dixon Fox et al., 2009 - Short- and long-range functions of Goosecoid in zebrafish axis formation are independent of Chordin, Noggin 1 and Follistatin-like 1b. Development (Cambridge, England)   136(10):1675-1685 Full text @ Development
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Antibody:
Fish:
Knockdown Reagents:
Anatomical Terms:
Stage Range: Shield to Prim-5
PHENOTYPE:
Fish:
Knockdown Reagents:
Observed In:
Stage: Prim-5

Fig. 7 gsc and chd morpholinos affect DV patterning. Injected construct or genotype is listed in lower right. (A-H) Live embryos, 1 dpf. (I-P) Tails, 1 dpf; stained with anti-Ntl antibody, anterior to the left. Arrowheads indicate thin notochords, arrows indicate truncated notochords. (Q-S) 60% epiboly embryos stained for wnt8; arrowhead marks dorsal. (Q) Control. (R) gsc-MO-injected embryo with ectopic wnt8 expression dorsally (11/81, 14%). (S) Ectopic staining in embryo injected with gsc-MO and a low concentration of chd-MO (46/106, 43%).

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
tbxta Ab1-ta AB standard conditions Prim-5 notochord IHC
Ab1-ta AB + MO1-chrd standard conditions Prim-5 notochord IHC
Ab1-ta AB + MO1-chrd + MO1-gsc standard conditions Prim-5 notochord IHC
Ab1-ta AB + MO1-gsc standard conditions Prim-5 notochord IHC
Ab1-ta chrdtt250/tt250 standard conditions Prim-5 notochord IHC
Ab1-ta chrdtt250/tt250 + MO1-gsc standard conditions Prim-5 notochord IHC
wnt8a AB + MO1-chrd + MO1-gsc standard conditions Shield margin ISH
AB + MO1-gsc standard conditions Shield margin ISH
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
AB + MO1-chrd standard conditions Prim-5 head decreased size, abnormal
Prim-5 notochord decreased width, abnormal
Prim-5 notochord truncated, abnormal
Prim-5 whole organism wholly ventralized, abnormal
AB + MO1-chrd + MO1-gsc standard conditions Prim-5 head decreased size, abnormal
Prim-5 notochord decreased width, abnormal
Prim-5 notochord truncated, abnormal
Prim-5 whole organism wholly ventralized, abnormal
AB + MO1-gsc standard conditions Prim-5 notochord morphology, normal
chrdtt250/tt250 standard conditions Prim-5 notochord decreased width, abnormal
Prim-5 whole organism wholly ventralized, abnormal
chrdtt250/tt250 + MO1-gsc standard conditions Prim-5 notochord truncated, abnormal
Prim-5 whole organism wholly ventralized, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Development (Cambridge, England) for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Development