ZFIN ID: ZDB-FIG-090501-26
Peterkin et al., 2009 - Common genetic control of haemangioblast and cardiac development in zebrafish. Development (Cambridge, England)   136(9):1465-1474 Full text @ Development
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Knockdown Reagents:
Anatomical Term:
Stage: 5-9 somites

Fig. 2 gata5 and gata6 rescue and gain-of-function experiments confirm necessity and identify sufficiency for myelopoiesis. Injection of exogenous gata5 and gata6 mRNA (g5+6RNA) into wild-type embryos did not induce fli1 or etsrp, whereas scl and pu1 were ectopically expressed. Rescue of all four genes was seen in gata5 and gata6 morphants co-injected with gata5 and gata6 mRNA (g5+6mo + g5+6RNA) when compared with gata5 and gata6 morphants alone (g5+6mo). Views are anterior-dorsal.

Gene Expression Details
Gene Antibody Fish Conditions Stage Qualifier Anatomy Assay
etsrp WT control 5-9 somites anterior lateral plate mesoderm ISH
WT + MO1-gata5 + MO1-gata6 standard conditions 5-9 somites anterior lateral plate mesoderm ISH
fli1 WT control 5-9 somites anterior lateral plate mesoderm ISH
WT + MO1-gata5 + MO1-gata6 standard conditions 5-9 somites anterior lateral plate mesoderm ISH
spi1b WT control 5-9 somites anterior lateral plate mesoderm ISH
WT + MO1-gata5 + MO1-gata6 standard conditions 5-9 somites Not Detected anterior lateral plate mesoderm ISH
tal1 WT control 5-9 somites anterior lateral plate mesoderm ISH
WT + MO1-gata5 + MO1-gata6 standard conditions 5-9 somites Not Detected anterior lateral plate mesoderm ISH
Antibody Labeling Details No data available
Phenotype Details No data available
Acknowledgments:
ZFIN wishes to thank the journal Development (Cambridge, England) for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Development