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ZFIN ID: ZDB-FIG-090408-30
Wilkinson et al., 2009 - Cep70 and Cep131 contribute to ciliogenesis in zebrafish embryos. BMC Cell Biology   10:17 Full text @ BMC Cell Biol.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Knockdown Reagent:
Anatomical Terms:
Stage: 90%-epiboly
PHENOTYPE:
Fish:
Knockdown Reagent:
Observed In:
Stage Range: Shield to 90%-epiboly

Fig. 6 Convergence-extension is unaffected in cep70 morphants. (A-F) Defective epiboly in cep70 morphant (cep131 morphants normal at this stage). At 70% epiboly, morphant embryos (B) have an acorn shape, unlike normal development at this stage (A). As epiboly completes, morphant embryos (D) (WT shown in C) extrude some of the yolk but will recover and survive. Assaying convergence-extension by the distance between anterior neural margin and prechordal plate by wholemount in situ hybridisation for dlx3 (purple) and hgg1 (light blue) reveals no difference between morphant (F) and WT (E). Scale bar: 125 μm.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
ctslb WT standard conditions 90%-epiboly prechordal plate ISH
WT + MO1-cep70 standard conditions 90%-epiboly prechordal plate ISH
dlx3b WT standard conditions 90%-epiboly presumptive neural plate ISH
WT + MO1-cep70 standard conditions 90%-epiboly presumptive neural plate ISH
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
WT + MO1-cep70 standard conditions Shield yolk protruding, abnormal
90%-epiboly yolk protruding, abnormal
Acknowledgments:
ZFIN wishes to thank the journal BMC Cell Biology for permission to reproduce figures from this article. Please note that this material may be protected by copyright.
Open Access.
Full text @ BMC Cell Biol.