Confocal wholemount (immuno)fluorescence localisation of Cep70 in zebrafish embryos. (A and E) In embryos injected with GFP-Cep70 and H2B-RFP, the Cep70 signal, though weak, is observed at the apical surface of cells in the otic vesicle (A) and eye (E) at 24 h.p.f. . Long dashed lines indicate the apical surface: interior to the circle in (A) and above the line in (E). Other panels: 24 h.p.f. zebrafish embryos were probed with anti-Cep70 (green) and either anti-acetylated or anti-gamma tubulin (red). (B, C, D) Otic vesicle stained for gamma tubulin (C), Cep70 (D) and merged picture (B) showing that the two signals coincide. Arrowheads point to the non-specific staining of the otoliths. (F) and (G) show the eye and otic vesicle; Cep70 can be seen at the base of cilia stained for acetylated-tubulin. (H) shows the region around one of the clusters of tether cells in the otic vesicle where the position of Cep70 can be seen more clearly; an inset shows one basal body and cilium. Yellow colour, the overlap of Cep70 and acetylated-tubulin signal is due to the angle at which some of the cilia are seen. Again, a long dashed line indicates the apical surface of the eye in (F). Short dashed lines delineate the otic vesicle in (B, C, D and G). (I) and (J) show Cep70 and cilia in the pronephros and spine canal. Again the Cep70 can be seen at the base of cilia. Orientation of the specimens with regard to the embryo is shown by the compass in the panel: A = anterior, P = posterior, D = dorsal, V = ventral. Scale bar: 20 μm.
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